PCNA AND KI67 EXPRESSION IN BREAST-CARCINOMA - CORRELATIONS WITH CLINICAL AND BIOLOGICAL VARIABLES
PCNA AND KI67 EXPRESSION IN BREAST-CARCINOMA - CORRELATIONS WITH CLINICAL AND BIOLOGICAL VARIABLES
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DOI：
10.1136/jcp.45.5.416
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发表时间：
1992-05-01
影响因子：
3.4
通讯作者：
BARBARESCHI, M
中科院分区：
文献类型：
--
作者：
LEONARDI, E;GIRLANDO, S;BARBARESCHI, M
目的：探讨两种细胞周期相关抗原（增殖细胞核抗原（PCNA）和Ki67相关抗原）在一系列乳腺癌中的表达情况；以及PCNA和Ki67标记指数（PCNA- li和Ki67- li）之间可能的相关性及其与其他生物学和临床病理变量的关联。方法：对96例导管癌和10例小叶癌进行分析。将样品固定在福尔马林和甲基萘甲酸中以定位PCNA。Ki67在冷冻切片上进行免疫染色。评估PCNA-LI和Ki67-LI与肿瘤大小、有丝分裂计数、组织学分级、淋巴结状态以及受体含量和p53基因表达改变的关系。结果：PCNA-LI与Ki67-LI没有相关性，也没有与任何其他变量相关。高KI67-LI（高于13-5的中位数）与高分级和有丝分裂计数、负受体含量和p53基因表达改变相关，但与其他变量无关。结论：PCNA-LI似乎不能替代Ki67-LI来评估乳腺癌的生长分数。
Aims: To investigate the expression of two cell cycle related antigens (proliferating cell nuclear antigen (PCNA) and Ki67 related antigen) in a series of breast cancers; and the possible correlations between the PCNA and Ki67 labelling indexes (PCNA-LI and Ki67-LI) and their associations with other biological and clinicopathological variables.Methods: Ninety six ductal and 10 lobular carcinoma specimens were investigated. Samples were fixed in formalin and in Methacarnoy for localisation of PCNA. Ki67 was immunostained on frozen sections. The PCNA-LI and Ki67-LI were evaluated in relation to tumour size, mitotic count, histological grade, nodal state as well as receptor content and altered expression of the p53 gene.Results: PCNA-LI did not correlate with Ki67-LI, nor was it associated with any other variable examined. A high KI67-LI (above the median value of 13-5) was associated with high grade and mitotic count, negative receptor content, and altered expression of the p53 gene, but not with other variables.Conclusions: The PCNA-LI does not seem to be a substitute for the Ki67-LI in evaluating the growth fraction in breast cancer.