HIV-1 Molecular Epidemiology, Transmission Clusters and Transmitted Drug Resistance Mutations in Central Brazil
HIV-1 Molecular Epidemiology, Transmission Clusters and Transmitted Drug Resistance Mutations in Central Brazil
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DOI：
10.3389/fmicb.2019.00020
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发表时间：
2019-01-31
影响因子：
5.2
通讯作者：
Coimbra Motta-Castro, Ana Rita
中科院分区：
文献类型：
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作者：
Ortiz Tanaka, Tayana Serpa;Leite, Thaysse Ferreira;Coimbra Motta-Castro, Ana Rita
我们的目的是表征HIV-1的分子流行病学和传播集群之间的异性恋（HET）和男性与男性发生性关系（MSM）的个人，以及传播耐药突变（TDRM）在巴西中西部。这项横断面调查是在190名未经抗逆转录病毒治疗的HIV-1感染者中进行的。提取前病毒DNA，巢式PCR扩增部分聚合酶基因（PR/RT）。测序后，分配亚型，并分析序列中可能的传播网络的出现。使用来自斯坦福大学HIV数据库的校准群体抗性（CPR）工具来研究TDRM的存在。在150个样本成功测序的个体中，最流行的HIV-1亚型是B，其次是重组形式。验证了至少由两个序列组成的20个传播簇的发生，表明来自相同或不同性取向的个体之间存在传播簇。在研究人群中发现中等水平的TDRM（12%），几乎一半的TDRM受试者有一个以上的耐药突变。未证实性取向与TDRM、HIV-1亚型/重组体形式之间存在相关性。总之，持续监测TDRM的必要性，以验证基因分型前的重要性，并描绘未来的策略，在主要的抗逆转录病毒治疗。同样，了解巴西的艾滋病毒-1传播网络将有助于在当地实施有效的艾滋病毒-1预防战略。
We aimed to characterize HIV-1 molecular epidemiology and transmission clusters among heterosexual (HET) and men who have sex with men (MSM) individuals, as well as transmitted drug resistance mutations (TDRM) in Central-Western Brazil. This cross-sectional survey was conducted among 190 antiretroviral naive HIV-1 infected individuals. Proviral DNA was extracted, and nested PCR amplified partial polymerase gene (PR/RT). After sequencing, subtypes were assigned, and the sequences were analyzed for the occurrence of possible transmission networks. Calibrated Population Resistance (CPR) tool from Stanford HIV Database was used to investigate the presence of TDRM. Among 150 individuals whose samples were successfully sequenced, the most prevalent HIV-1 subtype was B, followed by recombinant forms. The occurrence of twenty transmission clusters composed by at least two sequences was verified, suggesting the existence of transmission clusters among individuals from the same or distinct sexual orientations. Intermediate level of TDRM (12%) was found in the study population, and almost half of the subjects with TDRM had more than one resistance mutation. No correlations between sexual orientation and the presence of TDRM, HIV-1 subtypes/recombinants forms were verified. Taken together, the necessity of the continuous monitoring of the TDRM to verify the importance of pre-genotyping and to delineate future strategies in primary antiretroviral therapy. Likewise, the knowledge of the HIV-1 transmission networks in Brazil would allow the implementation of effective HIV-1 prevention strategies in local settings.