Underfeeding Alters Brain Tissue Synthesis Rate in a Rat Brain Injury Model.
Underfeeding Alters Brain Tissue Synthesis Rate in a Rat Brain Injury Model.
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DOI：
10.3390/ijms241713195
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发表时间：
2023-08-25
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
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作者：
脑损伤（BI）具有高度破坏性，通常具有长期持续的影响。医院中护理标准（SOC）能量支持不足导致BI患者的膳食能量缺乏。然而，目前还不清楚营养不足（UF）如何影响BI后的蛋白质合成。因此，在大鼠模型中，我们讨论了UF对BI后蛋白质合成率（fSR）的影响。与自由采食（AL）的动物相比，我们发现UF降低了后肢骨骼肌和皮质线粒体和结构蛋白的蛋白质合成（p ≤ 0.05）。与未损伤的假手术动物相比，BI显著增加了左右皮质的蛋白质合成（p ≤ 0.05），但抑制了小脑的蛋白质合成（p ≤ 0.05）。与单独喂养不足相比，UF与BI（UF+BI）联合导致皮质脑区域线粒体、胞质和全组织蛋白质的蛋白质合成速率增加。在UF+BI组中发现的蛋白质合成速率增加被AL喂养缓解，表明热量充足性证实了BI对皮质和小脑区域蛋白质动力学的影响。这项研究提供的证据表明，喂养不足对BI后的大脑愈合有负面影响，并且未受伤组织中的蛋白质储备被动员起来支持BI后的皮质组织修复。
Brain injuries (BI) are highly disruptive, often having long lasting effects. Inadequate standard of care (SOC) energy support in the hospital leads to dietary energy deficiencies in BI patients. However, it is unclear how underfeeding (UF) affects protein synthesis post-BI. Therefore, in a rat model, we addressed the issue of UF on the protein fractional synthesis rate (fSR) post-BI. Compared to ad libitum (AL)-fed animals, we found that UF decreased protein synthesis in hind-limb skeletal muscle and cortical mitochondrial and structural proteins (p ≤ 0.05). BI significantly increased protein synthesis in the left and right cortices (p ≤ 0.05), but suppressed protein synthesis in the cerebellum (p ≤ 0.05) as compared to non-injured sham animals. Compared to underfeeding alone, UF in conjunction with BI (UF+BI) caused increased protein synthesis rates in mitochondrial, cytosolic, and whole-tissue proteins of the cortical brain regions. The increased rates of protein synthesis found in the UF+BI group were mitigated by AL feeding, demonstrating that caloric adequacy alleviates the effects of BI on protein dynamics in cortical and cerebellar brain regions. This research provides evidence that underfeeding has a negative impact on brain healing post-BI and that protein reserves in uninjured tissues are mobilized to support cortical tissue repair following BI.
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DOI：
10.1093/function/zqab028
发表时间：
2021
期刊：
Function (Oxford, England)
影响因子：
--
作者：
Abbott CB;Lawrence MM;Kobak KA;Lopes EBP;Peelor FF 3rd;Donald EJ;Van Remmen H;Griffin TM;Miller BF
通讯作者：
Miller BF
影响因子：
4.2
作者：
Glenn TC;Martin NA;McArthur DL;Hovda DA;Vespa P;Johnson ML;Horning MA;Brooks GA
通讯作者：
Brooks GA
影响因子：
38.9
作者：
Moinard, C;Neveux, N;Cynober, L
通讯作者：
Cynober, L
DOI：
10.1164/ajrccm.151.6.7767529
发表时间：
1995-06-01
影响因子：
24.7
作者：
HOKANSON, JF;MERCIER, JG;BROOKS, GA
通讯作者：
BROOKS, GA
DOI：
10.1249/00005768-198606000-00019
发表时间：
1986-06-01
期刊：
MEDICINE AND SCIENCE IN SPORTS AND EXERCISE
影响因子：
--
作者：
BROOKS, GA
通讯作者：
BROOKS, GA