Spectroscopy of single hemoglobin molecules by surface enhanced Raman scattering
Spectroscopy of single hemoglobin molecules by surface enhanced Raman scattering
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DOI：
10.1103/physrevlett.83.4357
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发表时间：
1999-11-22
影响因子：
8.6
通讯作者：
Börjesson, L
中科院分区：
文献类型：
--
作者：
Xu, HX;Bjerneld, EJ;Börjesson, L
我们证明了在单个血红蛋白（Hb）的蛋白质分子连接到孤立的和固定的银纳米粒子的表面增强拉曼散射（Sers）的分子振动的检测。计算和实验之间的比较表明，电磁场效应占主导地位的表面增强，和单分子血红蛋白Sers是可能的，只有位于Ag颗粒之间的分子。的振动光谱表现出时间的波动来源不明，这似乎是单分子检测限的特性。
We demonstrate the detection of molecular vibrations in single hemoglobin (Hb) protein molecules attached to isolated and immobilized silver nanoparticles by surface enhanced Raman scattering (SERS). A comparison between calculation and experiment indicates that electromagnetic field effects dominate the surface enhancement, and that single molecule Hb SERS is possible only for molecules situated between Ag particles. The vibrational spectra exhibit temporal fluctuations of unknown origin which appear to be characteristic of the single molecule detection limit.