Housekeeping genes; expression levels may change with density of cultured cells
Housekeeping genes; expression levels may change with density of cultured cells
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DOI：
10.1016/j.jim.2010.02.006
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发表时间：
2010-04-15
影响因子：
2.2
通讯作者：
Raptis, Leda
中科院分区：
文献类型：
--
作者：
Greer, Samantha;Honeywell, Rice;Raptis, Leda
蛋白质印迹法是一种强大的技术来表征大量的细胞蛋白质。作为内部对照，印迹通常用于探测“管家”基因产物。在这篇文章中，我们发现细胞融合显著影响了两种广泛使用的蛋白质，α-微管蛋白和甘油醛-3-磷酸脱氢酶的水平。另一方面，热休克蛋白-90和β-肌动蛋白的水平在宽范围的细胞密度下保持不变，使这些蛋白质成为更可靠的上样对照。(C)2010 Elsevier B. V.保留所有权利。
Western blotting is a powerful technique to characterize a multitude of cellular proteins. As an internal control, the blots are commonly probed for "housekeeping" gene products. In this communication, we show that cell confluence significantly affects the levels of two such widely used proteins, alpha-tubulin and Glyceraldehyde-3-Phosphate Dehydrogenase. On the other hand the levels of heat-shock protein-90 and beta-actin remained unchanged at a wide range of cell densities, making these proteins into more reliable loading controls. (C) 2010 Elsevier B.V. All rights reserved.