Tissue oxygen saturation mapping with magnetic resonance imaging
Tissue oxygen saturation mapping with magnetic resonance imaging
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DOI：
10.1038/jcbfm.2014.116
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发表时间：
2014-09-01
影响因子：
6.3
通讯作者：
Barbier, Emmanuel Luc
中科院分区：
文献类型：
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作者：
Christen, Thomas;Bouzat, Pierre;Barbier, Emmanuel Luc
脑血氧饱和度的定量测定在脑血管疾病的研究中具有重要意义。虽然正电子发射断层扫描可以在体内绘制血液中的氧气水平，但它的可用性有限，并且需要电离辐射。磁共振成像（MRI）通过血氧水平依赖性对比提供了另一种选择。在这里，我们描述了一种在体内和非侵入性的方法来映射脑组织氧饱和度（StO（2））与高空间分辨率。通过MRI获得的StO（2）与各种氧气和红细胞压积挑战的血气分析结果相关性良好。在中风模型中，通过MRI在体内描绘的缺氧区域在空间上与通过匹莫硝唑染色在体外观察到的缺氧区域相匹配。在弥漫性创伤性脑损伤模型中，MRI甚至能够检测到StO（2）的减少，这种减少太小而无法通过组织学检测到。在F98胶质瘤模型中，MRI能够映射氧合异质性。因此，MRI技术可以提高我们对几种涉及氧合受损的脑部疾病的病理生理学的理解。
A quantitative estimate of cerebral blood oxygen saturation is of critical importance in the investigation of cerebrovascular disease. While positron emission tomography can Map in viva the oxygen level in blood, it has limited availability and requires ionizing radiation. Magnetic resonance imaging (MRI) offers an alternative through the blood oxygen level-dependent contrast. Here, we describe an in vivo and non-invasive approach to map brain tissue oxygen saturation (StO(2)) with high spatial resolution. StO(2) obtained with MRI correlated well with results from blood gas analyses for various oxygen and hematocrit challenges. In a stroke model, the hypoxic areas delineated in vivo by MRI spatially matched those observed ex vivo by pimonidazole staining. In a model of diffuse traumatic brain injury, MRI was able to-detect even a reduction in StO(2) that was too small to be detected by histology. In a F98 glioma model, MRI was able to map oxygenation heterogeneity. Thus, the MRI technique may improve our understanding of the pathophysiology of several brain diseases involving impaired oxygenation.