FREQUENT PRESENCE OF THE EPSTEIN-BARR-VIRUS IN INFLAMMATORY PSEUDOTUMOR
FREQUENT PRESENCE OF THE EPSTEIN-BARR-VIRUS IN INFLAMMATORY PSEUDOTUMOR
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DOI：
10.1016/0046-8177(95)90271-6
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发表时间：
1995-10-01
期刊：
影响因子：
3.3
通讯作者：
WEISS, LM
中科院分区：
文献类型：
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作者：
ARBER, DA;KAMEL, OW;WEISS, LM
炎性假瘤是一种可能发生在各种解剖部位的非肿瘤性、造血性和梭形纤维增生。这些扩散的起源一般是未知的。为探讨EB病毒（EBV）在炎性假瘤中的作用，应用原位杂交技术对17例18例炎性假瘤进行了EBV RNA的检测，并对感染细胞的形态学和免疫学特征进行了评价。这些标本包括10个淋巴结，6个脾肿块和2个肝肿块。总体而言，在41.2%（18例中的7例）的病例中检测到EBV RNA。其中包括10个淋巴结中的2个（20%）、6个脾假瘤中的4个（66.7%）和2个肝脏病变中的1个（50%）。与周围未受累组织相比，肿瘤内EBV感染的程度明显更高。两种形态学上不同的EBV阳性细胞类型，梭形和圆形细胞，是显而易见的，和感染的细胞类型显着不同时，淋巴结和淋巴结的情况进行了比较。所有阳性淋巴结病例均显示大量EBV阳性梭形细胞，而在两个EBV阳性淋巴结假瘤中未观察到阳性梭形细胞（仅阳性圆形细胞，形态学上与淋巴细胞一致）。双标记免疫组化和原位杂交研究在某些情况下确定了罕见的EBV阳性B细胞和罕见的EBV阳性T细胞分别在4例和3例。所有病例中的大多数EBV阳性细胞均未与任何B或T细胞标记物发生免疫反应。然而，研究的五个病例中有三个确实显示了平滑肌肌动蛋白/EBV阳性梭形细胞的亚群，七个病例中有五个显示了波形蛋白/EBV阳性梭形细胞，四个病例中有一个有EBV阳性梭形细胞，其免疫反应为滤泡树突状细胞。这些结果表明，EBV在炎性假瘤的显着数量的情况下，EBV感染的发病率和细胞类型（梭形与圆形细胞）感染的差异，当淋巴结和淋巴结的情况下进行比较，表明发病机制的差异。结节性病例中感染的细胞类型似乎是间充质来源，但不能明确界定。版权所有（C）1995由W.B.桑德斯公司
Inflammatory pseudotumor is a presumably nonneoplastic, hematopoietic, and spindled fibrous proliferation that may occur at a variety of anatomic sites. The origin of these proliferations is generally unknown. To evaluate the role of the Epstein-Barr virus (EBV) in inflammatory pseudotumor, 18 specimens from 17 patients were studied by in situ hybridization for EBV ribonucleic acid (RNA), and the morphological and immunologic characteristics of the infected cells were evaluated. These specimens included 10 lymph nodes, six splenic masses, and two hepatic masses. Overall, EBV RNA was detected in 41.2% (seven of 18) of the cases. These included two of 10 (20%) lymph nodes, four of six (66.7%) splenic pseudotumors, and one of two (50%) hepatic lesions. The degree of EBV infection was significantly greater within the tumors in comparison with the surrounding, uninvolved tissue. Two morphologically different EBV-positive cell types, spindled and round cells, were evident, and the infected cell type differed significantly when the nodal and extranodal cases were compared. All of the positive extranodal cases shown, numerous EBV-positive spindled cells, whereas no positive spindle cells (only positive round cells, morphologically consistent with lymphocytes) were noted in the two EBV-positive lymph node pseudotumors. Double-labeling immunohistochemical and in situ hybridization studies in some cases identified rare EBV-positive B cells and rare EBV positive T cells in four and three cases, respectively. Most EBV-positive cells in all cases failed to immunoreact with any B- or T-cell markers. Three of five cases studied, however, did show a subpopulation of smooth muscle actin/EBV-positive spindled cells, five of seven cases showed vimentin/EBV-positive spindled cells, and one of four cases had EBV-positive spindled cells that inmunoreacted as follicular dendritic cells. These results suggest that EBV plays a role in a significant number of cases of inflammatory pseudotumor with differences in the incidence of EBV infection and the cell type (spindled vs round cell) infected when extranodal and nodal cases are compared, suggesting a difference in pathogenesis. The cell type infected in extranodal eases seemed to be of mesenchymal origin but could not be clearly defined. Copyright (C) 1995 by W.B. Saunders Company