Glycoconjugate vaccine containing Escherichia coli O157:H7 O-antigen linked with maltose-binding protein elicits humoral and cellular responses.
Glycoconjugate vaccine containing Escherichia coli O157:H7 O-antigen linked with maltose-binding protein elicits humoral and cellular responses.
复制标题
含有与麦芽糖结合蛋白连接的大肠杆菌 O157:H7 O 抗原的糖复合疫苗可引发体液和细胞反应
DOI：
10.1371/journal.pone.0105215
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Chen M
中科院分区：
文献类型：
--
作者：
Ma Z;Zhang H;Shang W;Zhu F;Han W;Zhao X;Han D;Wang PG;Chen M
Glycoconjugate is one of the most efficacious and safest vaccines against bacterial pathogens. Previous studies of glycoconjugates against pathogen E. coli O157:H7 focused more on the humoral responses they elicited. However, little was known about their cellular responses. In this study, we exploited a novel approach based on bacterial protein N-linked glycosylation system to produce glycoconjugate containing Escherichia coli O157:H7 O-antigen linked with maltose-binding protein and examined its humoral and cellular responses in BALB/c mice. The transfer of E. coli O157:H7 O-antigen to MBP was confirmed by western blot and MALDI-TOF MS. Mice injected with glycoconjugate O-Ag-MBP elicited serum bactericidal antibodies including anti-E. coli O157:H7 O-antigen IgG and IgM. Interestingly, O-Ag-MBP also stimulated the secretion of anti-E. coli O157:H7 O-antigen IgA in intestine. In addition, O-Ag-MBP stimulated cellular responses by recruiting Th1-biased CD4+ T cells, CD8+ T cells. Meanwhile, O-Ag-MBP induced the upregulation of Th1-related IFN-γ and downregulation of Th2-related IL-4, and the upregulation of IFN-γ was stimulated by MBP in a dose-dependent manner. MBP showed TLR4 agonist-like properties to activate Th1 cells as carrier protein of O-Ag-MBP. Thus, glycoconjugate vaccine E. coli O157:H7-specific O-Ag-MBP produced by bacterial protein N-linked glycosylation system was able to elicit both humoral and Th1-biased cellular responses.
登录
查看更多内容
影响因子：
6.4
作者：
Iwashkiw JA;Fentabil MA;Faridmoayer A;Mills DC;Peppler M;Czibener C;Ciocchini AE;Comerci DJ;Ugalde JE;Feldman MF
通讯作者：
Feldman MF
影响因子：
11.2
作者：
Rossi, GR;Mautino, MR;Link, CJ
通讯作者：
Link, CJ
影响因子：
3.1
作者：
Fernandez, Stefan;Palmer, Dupeh R.;Sun, Wellington
通讯作者：
Sun, Wellington
DOI：
10.1073/pnas.0500044102
发表时间：
2005-02-22
影响因子：
11.1
作者：
Feldman, MF;Wacker, M;Aebi, M
通讯作者：
Aebi, M
影响因子：
6.2
作者：
Lockhart, Stephen
通讯作者：
Lockhart, Stephen