The expression profile of purified Drosophila germline stem cells
The expression profile of purified Drosophila germline stem cells
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DOI：
10.1016/j.ydbio.2005.04.018
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发表时间：
2005-07-15
影响因子：
2.7
通讯作者：
Spradling, AC
中科院分区：
文献类型：
--
作者：
Kai, T;Williams, D;Spradling, AC
我们开发了一种从果蝇卵巢高度纯化生殖系干细胞（GSC）的方法，这是最好理解的成体干细胞类型之一。GSC表达一般转录组分的变体同种型、翻译起始因子和几种变体核糖体蛋白，包括RpL 22，一种在几种哺乳动物干细胞中富集的蛋白。这些新的同种型可能有助于调节干细胞基因表达，因为逆转试验表明至少有四种对GSC特异。通过比较分析，我们确定了GSC中富集的其他基因，包括Psc，Bmi-1 Polycomb组基因的果蝇同源物，以及可能延迟减数分裂前生殖细胞胞质分裂的基因。通过比较BMP过表达和bam突变所抑制的GSC，我们假设mRNA的利用在分化GSC子代中受到调节。我们的研究结果表明，果蝇和哺乳动物干细胞利用至少两个共同的调节机制。(c)2005年由Elsevier Inc.出版
We developed a method to highly purify germline stem cells (GSCs) from the Drosophila ovary, one of the best understood types of adult stem cell. GSCs express variant isoforms of general transcriptional components, translation initiation factors, and several variant ribosomal proteins, including RpL22, a protein enriched in several mammalian stem cells. These novel isoforms may help regulate stem cell gene expression because a reversion assay indicated that at least four were specific for GSCs. By comparative analysis, we identify additional genes enriched in GSCs, including Psc, the Drosophila homolog of the Bmi-1 Polycomb group gene, as well as genes that may delay cytokinesis in pre-meiotic germ cells. By comparing GSCs arrested by BMP over-expression and bam mutation, we hypothesize that mRNA utilization is modulated in differentiating GSC daughters. Our findings suggest that Drosophila and mammalian stem cells utilize at least two regulatory mechanisms in common. (c) 2005 Published by Elsevier Inc.