The Drosophila Hem/Kette/Nap1 protein regulates asymmetric division of neural precursor cells by regulating localization of Inscuteable and Numb.
The Drosophila Hem/Kette/Nap1 protein regulates asymmetric division of neural precursor cells by regulating localization of Inscuteable and Numb.
复制标题
果蝇 Hem/Kette/Nap1 蛋白通过调节 Inscuteable 和 Numb 的定位来调节神经前体细胞的不对称分裂。
DOI：
10.1016/j.mod.2011.09.004
复制
发表时间：
2011
影响因子：
2.6
通讯作者：
Bhat,KrishnaMoorthi
中科院分区：
文献类型：
--
作者：
Zhu,Zengrong;Bhat,KrishnaMoorthi
Hem/Kette/Nap 1蛋白参与许多生物过程。我们最近报道，在果蝇胚胎神经元的正常迁移所需的Hem。在本文中，我们报告，Hem调节神经前体细胞的不对称分裂。我们发现，一个充分研究的Hem/Kette突变等位基因产生至少两个主要的，但可能更多的，表型类的突变胚胎，这些表型与可变水平的母体野生型Hem蛋白在发育中的胚胎。而较弱的类别表现出弱的轴突导向缺陷和神经元的误迁移，较强的类别导致严重的轴突导向缺陷，神经元的误迁移和对称分裂的RP 2/同胞谱系的神经节母细胞（GMC）。我们还表明，损失的不对称分裂的基础是由于非本地化的GMC-1中的Inscuteable和Numb。非不对称Numb分离到GMC-1的两个子细胞，然后阻止Notch信号传导指定同胞命运。这导致两种细胞都采用RP 2命运。此外，Abelson酪氨酸激酶的功能丧失还导致Insuteable和Numb的不对称定位的丧失以及GMC-1的对称分裂，WAVE的功能丧失具有非常弱的不对称缺陷的渗透性丧失。这些结果定义了Hem/Kette/Nap 1在神经发生过程中在神经前体细胞中的另一个作用。
The Hem/Kette/Nap1 protein is involved in many biological processes. We have recently reported that Hem is required for the normal migration of neurons in the Drosophila embryo. In this paper, we report that Hem regulates the asymmetric division of neural precursor cells. We find that a well-studied Hem/Kette mutant allele produces at least two main, but possibly more, phenotypic classes of mutant embryos, and these phenotypes correlate with variable levels of maternal wild type Hem protein in the developing embryo. While the weaker class exhibits weak axon guidance defect and the mis-migration of neurons, the stronger class causes severe axon guidance defects, mis-migration of neurons and symmetric division of ganglion mother cells (GMC) of the RP2/sib lineage. We also show that the basis for the loss of asymmetric division is due to non-localization of Inscuteable and Numb in GMC-1. A non-asymmetric Numb segregates to both daughter cells of GMC-1, which then prevents Notch signaling from specifying a sib fate. This causes both cells to adopt an RP2 fate. Furthermore, loss of function for Abelson tyrosine kinase also causes loss of asymmetric localization of Inscuteable and Numb and symmetric division of GMC-1, the loss of function for WAVE has a very weakly penetrant loss of asymmetry defect. These results define another role for Hem/Kette/Nap1 in a neural precursor cell during neurogenesis.