Plasma homocysteine concentrations in novel microminipigs.
Plasma homocysteine concentrations in novel microminipigs.
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发表时间：
2014-07
期刊：
影响因子：
2.3
通讯作者：
Toshiaki Kakimoto;Akira Otsuka;H. Kawaguchi;K. Ogata;A. Tanimoto;H. Kanouchi
中科院分区：
文献类型：
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作者：
Toshiaki Kakimoto;Akira Otsuka;H. Kawaguchi;K. Ogata;A. Tanimoto;H. Kanouchi
最近开发了一种用于生物医学研究的新型微型猪。在本研究中，研究了这些小型猪的年龄和性别差异，以及血浆总同型半胱氨酸浓度(THcy)的24小时波动。THcy(平均值±SD)为10.2±3.4μM，与年龄显著相关。相比之下，tHcy的性别差异和喂养后24小时的tHcy波动均不显著。静脉注射还原型Hcy后血浆tHcy的动态变化显示其水平在注射后5min达到峰值，tHcy的水平也在注射后5min内达到峰值。这些结果表明还原的同型半胱氨酸被迅速氧化或代谢。血中还原同型半胱氨酸、总同型半胱氨酸和半胱氨酸的半衰期分别为47、71和141min。综上所述，小型猪的年龄与血浆tHcy呈显著正相关。静脉注射还原同型半胱氨酸后，血浆总同型半胱氨酸迅速恢复到注射前水平。
A novel microminipig has been recently developed for use in biomedical research. In the present study, age- and sex-related differences, as well as 24-h fluctuations in plasma total homocysteine concentrations (tHcy), were investigated in these microminipigs. tHcy (mean±SD) was 10.2±3.4 μM and significantly correlated with age. By contrast, neither the differences in tHcy between sexes nor the 24-h fluctuations in tHcy after feeding were significant. The kinetics of plasma tHcy after intravenous injection of reduced Hcy showed that its levels peaked within 5 min post-injection, as did the levels of tHcy. These results suggested that reduced Hcy is rapidly oxidized or metabolized. The half-lives of reduced Hcy, tHcy, and reduced cysteine in the blood were 47, 71, and 141 min, respectively. In conclusion, there was a significantly positive correlation between age and plasma tHcy in microminipigs. After intravenous injection of reduced Hcy, plasma tHcy quickly returned to pre-injection levels.