β-Cryptoxanthin from Citrus juices:: assessment of bioaccessibility using an in vitro digestion/Caco-2 cell culture model
β-Cryptoxanthin from Citrus juices:: assessment of bioaccessibility using an in vitro digestion/Caco-2 cell culture model
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DOI：
10.1017/s0007114507670822
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发表时间：
2007-05-01
影响因子：
3.6
通讯作者：
Amiot, Marie-Josephe
中科院分区：
文献类型：
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作者：
Dhuique-Mayer, Claudie;Borel, Patrick;Amiot, Marie-Josephe
β-隐黄质（β-CX）是一种对健康具有潜在意义的维生素原类胡萝卜素，主要以游离和酯化形式存在于柑橘类水果中。关于β-CX的肠吸收，特别是酯化形式的肠吸收知之甚少。本研究的目的是使用模拟消化结合Caco-2模型评价游离和酯化β-CX的吸收。利用体外消化系统，通过测定不同柑橘类果汁中类胡萝卜素向胶束中的转移来研究其生物可及性。然后，通过将富含类胡萝卜素的胶束（来自体外消化）或合成胶束（由合成脂质和从柑橘汁纯化的类胡萝卜素制成）加入人肠细胞（Caco-2 TC 7克隆）来评估类胡萝卜素摄取。我们的结果表明，在体外消化过程中，β-隐黄素酯（β-CXE）被部分水解。测得的游离β-CX的生物可利用性（40（SD 1.05）%）显著高于β-胡萝卜素（30（SD 1.9）%）和β-CXE（16（SD 1.5）%）。以同样的方式，游离β-CX（27（SD 1.01）%）掺入合成胶束超过（P
beta-Cryptoxanthin (beta-CX), a provitaminic carotenoid of potential interest for health, is found principally in Citrus fruit in both free and esterified forms. Little is known about the intestinal absorption of beta-CX especially with regard to the esterified forms. The aim of this study was to evaluate the absorption of free and esterified beta-CX using simulated digestion coupled with the Caco-2 model. Bioaccessibility was investigated by measuring the transfer of carotenoids from different citrus juices into micelles using an in vitro digestion system. Then, carotenoid uptake was evaluated by adding carotenoid-rich micelles (from the in vitro digestion) or synthetic micelles (made from synthetic lipids and carotenoids purified from citrus juice) to human intestinal cells (Caco-2 TC7 clone). Our results showed that beta-cryptoxanthin esters beta-CXE) were partially hydrolysed during the in vitro digestion. The bioaccessibility of free beta-CX measured was significantly higher (40 (SD 1.05) %) than that of beta-carotene (30 (SD 1.9) %) and (beta-CXE (16 (SD 1.5) %). In the same way, the incorporation of free beta-CX (27 (SD 1.01) %) into synthetic micelles exceeded (P