APPLICATION OF PYROLYSIS MASS-SPECTROSCOPY AND SDS-PAGE IN THE STUDY OF THE EPIDEMIOLOGY OF PSEUDOMONAS-CEPACIA IN CYSTIC-FIBROSIS
APPLICATION OF PYROLYSIS MASS-SPECTROSCOPY AND SDS-PAGE IN THE STUDY OF THE EPIDEMIOLOGY OF PSEUDOMONAS-CEPACIA IN CYSTIC-FIBROSIS
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DOI：
10.1099/00222615-41-2-106
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发表时间：
1994-08-01
影响因子：
3
通讯作者：
HART, CA
中科院分区：
文献类型：
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作者：
CORKILL, JE;SISSON, PR;HART, CA
通过SDS-PAGE全细胞多肽和热解质谱（PMS）研究了来自桤木Hey儿童医院呼吸科的15名囊性纤维化（CF）患者的洋葱假单胞菌代表性分离株。SDS-PAGE的区分度低于PMS。11个分离株是不可区分的PMS，并认为代表再分离的地方性菌株;四个分离株是不同的，从这个组，从另一个。洋葱青霉菌于1989年7月首次在该单位从一名参加加拿大CF营地英国选拔会议的患者身上分离出来。一个病房和门诊隔离政策被引入，但殖民化的进一步病人发生。1991年8月，成人CF协会建议所有涉及殖民地患者的社交活动都应停止。这一点，以及老年CF患者对人与人之间传播风险的认识提高，与新病例的显着下降有关。在洋葱疫霉定植前一年，对定植患者的社会活动和住院情况进行了比较，并与未获得地方性菌株的患者进行了匹配。这表明，殖民化患者在CF社交活动中的出席率显着较高，但殖民化和住院之间没有显着关联。这些结果是洋葱疫霉通过医院环境外的社会接触传播的有力间接证据。
Representative isolates of Pseudomonas cepacia from 15 cystic fibrosis (CF) patients attending the Respiratory Unit of Alder Hey Childrens' Hospital were investigated by SDS-PAGE of whole-cell polypeptides and by pyrolysis mass spectroscopy (PMS). SDS-PAGE was less discriminatory than PMS. Eleven isolates were indistinguishable by PMS and considered to represent re-isolates of an endemic strain; four isolates were distinct from this group, and from one another. P. cepacia was first isolated on the unit in July 1989 from a patient who had attended a UK selection meeting for a Canadian CF camp. A ward and outpatient segregation policy was introduced, but colonisation of further patients occurred. In August 1991, the Adult CF Association recommended that all social activities involving colonised patients should cease. This, and an increased awareness amongst older CF patients of the risks of person-to-person transmission, was associated with a marked decline in new cases. Social activity and hospital admissions were compared for colonised patients during the year before colonisation with P. cepacia, and matched patients who did not acquire the endemic strain. This showed a significantly higher attendance at CF social events for colonised patients, but no significant association between colonisation and hospital admission. These results are strong indirect evidence that transmission of P. cepacia occurs through social contact outside the hospital environment.