Enzyme Release from Polyion Complex by Extremely Low Frequency Magnetic Field.
Enzyme Release from Polyion Complex by Extremely Low Frequency Magnetic Field.
复制标题
通过极低频磁场从聚离子复合物中释放酶。
DOI：
10.1038/s41598-020-61364-w
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发表时间：
2020
影响因子：
4.6
通讯作者：
Kabanov,AlexanderV
中科院分区：
文献类型：
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作者：
Vlasova,KseniyaYu;Vishwasrao,Hemant;Abakumov,MaximA;Golovin,DmitryYu;Gribanovsky,SergeyL;Zhigachev,AlexanderO;Poloznikov,AndreyА;Majouga,AlexanderG;Golovin,YuriI;Sokolsky-Papkov,Marina;Klyachko,NataliaL;Kabanov,AlexanderV
通过非加热极低频磁场（ELF MF）对磁性纳米颗粒（MNP）的远程纳米磁机械驱动被探索作为在制药和医疗应用中对生物纳米材料进行非侵入性改性的工具。在这里，我们研究了ELF MF（30-160 Hz，8-120 kA/m）对模型酶超氧化物歧化酶1（SOD 1）的活性和释放的影响，超氧化物歧化酶1（SOD 1）通过聚离子偶联固定在分散的MNP聚集体上，该聚集体涂覆有聚（L-赖氨酸）-嵌段-聚（乙二醇）嵌段共聚物（s-MNP）。这样的场不会引起MNP的任何相当大的加热，但会促进它们的振荡机械运动，从而在相邻材料中产生机械力和变形。我们观察了固定化SOD 1的催化活性的变化，以及其从s-MNP/SOD 1聚离子复合物的应用ELF MF 5至15分钟后释放。在较长的暴露（25分钟）下，s-MNP/SOD 1分散体不稳定。本文报道并解释了最大场强为50 Hz时的钟形效应和场强为30 ~ 120 kA/m时的饱和效应。这些发现具有重要意义，是ELF MF对协同聚离子复合物进行纳米磁机械破坏的早期迹象，这些复合物广泛用于当前功能性医疗保健生物纳米材料的设计。
Remote nano-magneto-mechanical actuation of magnetic nanoparticles (MNPs) by non-heating extremely low frequency magnetic field (ELF MF) is explored as a tool for non-invasive modification of bionanomaterials in pharmaceutical and medical applications. Here we study the effects of ELF MF (30–160 Hz, 8–120 kA/m) on the activity and release of a model enzyme, superoxide dismutase 1 (SOD1) immobilized by polyion coupling on dispersed MNPs aggregates coated with poly(L-lysine)-block-poly(ethylene glycol) block copolymer (s-MNPs). Such fields do not cause any considerable heating of MNPs but promote their rotating-oscillating mechanical motion that produces mechanical forces and deformations in adjacent materials. We observed the changes in the catalytic activity of immobilized SOD1 as well as its release from the s-MNPs/SOD1 polyion complex upon application of the ELF MF for 5 to 15 min. At longer exposures (25 min) the s-MNPs/SOD1 dispersion destabilizes. The bell-shaped effect of the field frequency with maximum atf= 50 Hz and saturation effect of field strength (between 30 kA/m and 120 kA/m atf= 50 Hz) are reported and explained. The findings are significant as one early indication of the nano-magneto-mechanical disruption by ELF MF of cooperative polyion complexes that are widely used for design of current functional healthcare bionanomaterials.