Region-based diffuse optical tomography with registered atlas: in vivo acquisition of mouse optical properties.
Region-based diffuse optical tomography with registered atlas: in vivo acquisition of mouse optical properties.
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DOI：
10.1364/boe.7.005066
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发表时间：
2016-12
影响因子：
3.4
通讯作者：
Wenbo Wan;Yihan Wang;Jin Qi;Lingling Liu;Wenjuan Ma;Jiao Li;Limin Zhang;Zhong-xing Zhou;Huijuan Zhao;F. Gao
中科院分区：
文献类型：
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作者：
Wenbo Wan;Yihan Wang;Jin Qi;Lingling Liu;Wenjuan Ma;Jiao Li;Limin Zhang;Zhong-xing Zhou;Huijuan Zhao;F. Gao
在基于模型的光学层析成像模式的重建质量可以大大受益于准确的组织光学特性的先验信息，这是很难在体内获得与传统的扩散光学层析成像（DOT）系统单独。解决方案之一是应用利用诸如X射线计算机断层扫描（XCT）的解剖成像系统获得的先验解剖结构来约束DOT的重建过程。然而，由于X射线提供低的软组织对比度，因此从单独的XCT图像分割腹部器官可能是有问题的。为了克服这些挑战，本研究提出了一种恢复先验器官导向组织光学特性的新方法，其中通过配准标准解剖图谱近似获得体内小鼠的解剖结构，即，Digimouse与目标XCT体积进行非刚性图像配准，并进而用于引导DOT提取内部器官的光学特性。模拟研究已经验证了这种基于图谱配准的DOT策略在揭示先验解剖结构和光学特性方面的方法学可用性。进一步的实验证明了该方法用于获取活体小鼠器官组织光学特性的可行性，使其成为一种有效的重建增强方法。
The reconstruction quality in the model-based optical tomography modalities can greatly benefit from a priori information of accurate tissue optical properties, which are difficult to be obtained in vivo with a conventional diffuse optical tomography (DOT) system alone. One of the solutions is to apply a priori anatomical structures obtained with anatomical imaging systems such as X-ray computed tomography (XCT) to constrain the reconstruction process of DOT. However, since X-ray offers low soft-tissue contrast, segmentation of abdominal organs from sole XCT images can be problematic. In order to overcome the challenges, the current study proposes a novel method of recovering a priori organ-oriented tissue optical properties, where anatomical structures of an in vivo mouse are approximately obtained by registering a standard anatomical atlas, i.e., the Digimouse, to the target XCT volume with the non-rigid image registration, and, in turn, employed to guide DOT for extracting the optical properties of inner organs. Simulative investigations have validated the methodological availability of such atlas-registration-based DOT strategy in revealing both a priori anatomical structures and optical properties. Further experiments have demonstrated the feasibility of the proposed method for acquiring the organ-oriented tissue optical properties of in vivo mice, making it as an efficient way of the reconstruction enhancement.