Mucosa-associated bacteria in the human gastrointestinal tract are uniformly distributed along the colon and differ from the community recovered from feces
Mucosa-associated bacteria in the human gastrointestinal tract are uniformly distributed along the colon and differ from the community recovered from feces
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DOI：
10.1128/aem.68.7.3401-3407.2002
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发表时间：
2002-07-01
影响因子：
4.4
通讯作者：
de Vos, WM
中科院分区：
文献类型：
--
作者：
Zoetendal, EG;von Wright, A;de Vos, WM
人胃肠道（GI）在粘膜、管腔和粪便中含有复杂的细菌细胞群落。由于大多数注意力都集中在粪便中存在的细菌上，因此有关结肠不同部位粘膜相关细菌群落的知识有限。在这项研究中，通过使用16S rRNA方法分析了10个个体的升、横和降结肠的粪便和活检样本中的细菌群落。流式细胞术分析表明，105至106个细菌存在于活检样本。为了直观的多样性的优势和乳酸菌群社区，变性梯度凝胶电泳（DGGE）分析16S rRNA基因扩增子进行。DGGE分析和相似性指数比较表明，肠道粘膜相关细菌优势菌群具有宿主特异性，沿结肠沿着分布均匀，但与粪便菌群差异显著（P < 0.01）。乳杆菌属特定群的概况不如反映主要群落的概况复杂。对于10个个体中的6个，活检样品中的乳杆菌样细菌群落与粪便中的相似。在9个个体的活检样品中检测到与加氏乳杆菌的16S核糖体DNA具有99%序列相似性的扩增子。在健康和患病个体之间没有观察到显著差异。观察到的宿主特异性DGGE曲线的粘膜相关的细菌群落在结肠支持的假设，即宿主相关的因素参与了胃肠道微生物群落的测定。
The human gastrointestinal (GI) tract harbors a complex community of bacterial cells in the mucosa, lumen, and feces. Since most attention has been focused on bacteria present in feces, knowledge about the mucosa-associated bacterial communities in different parts of the colon is limited. In this study, the bacterial communities in feces and biopsy samples from the ascending, transverse, and descending colons of 10 individuals were analyzed by using a 16S rRNA approach. Flow cytometric analysis indicated that 105 to 106 bacteria were present in the biopsy samples. To visualize the diversity of the predominant and the Lactobacillus group community, denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene amplicons was performed. DGGE analysis and similarity index comparisons demonstrated that the predominant mucosa-associated bacterial community was host specific and uniformly distributed along the colon but significantly different from the fecal community (P < 0.01). The Lactobacillus group-specific profiles were less complex than the profiles reflecting the predominant community. For 6 of the 10 individuals the community of Lactobacillus-like bacteria in the biopsy samples was similar to that in the feces. Amplicons having 99% sequence similarity to the 16S ribosomal DNA of Lactobacillus gasseri were detected in the biopsy samples of nine individuals. No significant differences were observed between healthy and diseased individuals. The observed host-specific DGGE profiles of the mucosa-associated bacterial community in the colon support the hypothesis that host-related factors are involved in the determination of the GI tract microbial community.