Splenectomy, suppressor cell activity, and survival in tumor bearing rats.
Splenectomy, suppressor cell activity, and survival in tumor bearing rats.
复制标题
脾切除术、抑制细胞活性和荷瘤大鼠的存活。
DOI：
10.1016/0022-4804(80)90021-9
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
J. Meyer
中科院分区：
文献类型：
--
作者：
J. Meyer;B. Argyris;J. Meyer
10周龄Fischer品系雌性幼鼠接受脾切除手术。三周后，这些动物和一组可比的完整动物被接种于同基因Ward结肠癌的3×106细胞皮下。连续测量肿瘤生长情况。来自对照、完整荷瘤动物和脾肿瘤动物的淋巴结细胞在混合淋巴细胞培养(MLC)中的反应性和抑制同种异体混合淋巴细胞反应的能力被测试。接种肿瘤后3周，荷瘤宿主脾淋巴细胞反应性有所降低，而正常宿主淋巴细胞反应性明显降低(P<；0.001)，正常宿主淋巴细胞抑制活性明显高于正常宿主(P<；0.001)。5周时，两组MLC中淋巴细胞反应性均受到严重抑制；抑制性细胞活性均已存在，差异无统计学意义。完整荷瘤动物接种后的中位生存期为97天(经对数等级检验P<；0.0001)，脾动物的中位生存期为120天(P<；0.001)。我们的结论是，脾肿瘤动物淋巴细胞反应性的抑制和抑制活性的开始都是延迟的。寄主存活时间显著延长。
Young female rats of the Fischer strain were subjected to splenectomy at 10 weeks of age. Three weeks later, these animals and a comparable group of intact animals were inoculated subcutaneously with 3 × 106cells of the syngeneic Ward colon carcinoma. Tumor growth was recorded by serial measurement. Lymph node cells from control, intact tumor-bearing, and asplenic tumor-bearing animals were tested for reactivity in mixed lymphocyte culture (MLC) and for ability to suppress the allogeneic mixed lymphocyte reaction. At 3 weeks after tumor inoculation, lymphocyte reactivity in MLC was somewhat depressed in asplenic tumor-bearing hosts but severely depressed in intact hosts (P< 0.001); suppressor activity was demonstrable in intact hosts but not in asplenic hosts (P< 0.001). At 5 weeks, lymphocyte reactivity in MLC was severely depressed in both groups; suppressor cell activity was present in both groups and differences were no longer significant. Median survival of intact tumor-bearing animals was 97 days after inoculation (P< 0.0001 by the log-rank test), and 120 days in asplenic animals (P< 0.001). We conclude that both depression of lymphocyte reactivity and onset of suppressor activity are delayed in asplenic tumor-bearing animals. Host survival was significantly prolonged.