Diminazene aceturate in the control of Trypanosoma evansi infection in cats
Diminazene aceturate in the control of Trypanosoma evansi infection in cats
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DOI：
10.1016/j.vetpar.2009.06.025
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发表时间：
2009-10-28
影响因子：
2.6
通讯作者：
Monteiro, S. G.
中科院分区：
文献类型：
--
作者：
Da Silva, A. S.;Zanette, R. A.;Monteiro, S. G.
本研究的目的是研究二氨基氮烯乙酸酯在控制猫伊氏锥虫感染方面的功效。十四只动物每只感染 10(8) 种锥鞭毛体形式，六只动物用作阴性对照（A 组）。七只受感染的猫用作阳性对照（B 组），七只用二氨基氮烯乙酸酯（3.5 mg kg(-1)）或连续 5 天治疗（C 组）。在实验过程中评估生化和血液学参数。接种后第49天收集含有抗凝剂的血液并保存在乙醇中用于DNA提取。使用伊氏锥虫特异性 PCR 对样本进行分析，以评估治疗的有效性。用二氨基氮烯乙酸酯治疗有效率85.7%。接受治疗的猫的丙氨酸氨基转移酶、γ-谷氨酰转移酶、尿素和肌酐值保持在正常生理范围内。所有治愈动物的血象均正常化。因此，所使用的疗法可有效控制猫的伊氏锥虫。 (C) 2009 Elsevier B.V. 保留所有权利。
The aim of this study was to investigate the efficacy of diminazene aceturate in the control of the infection by Trypanosoma evansi in cats. Fourteen animals were infected with 10(8) trypomastigote forms each and six were used as negative control (group A). Seven of the infected cats were used as positive control (group B) and seven were treated with diminazene aceturate (3.5 mg kg(-1)) or 5 consecutive days (group C). Biochemical and hematological parameters were evaluated during the experiment. Blood with anticoagulant was collected at day 49 post-inoculation and preserved in ethanol for DNA extraction. Samples were analyzed using PCR T. evansi-specific to assess the effectiveness of treatment. The treatment with diminazene aceturate had an efficacy of 85.7%. Alanine aminotransferase, gamma-glutamyltransferase, urea, and creatinine values remained within the normal physiological range in the treated cats. Hemogram was normalized in all the cured animals. Therefore, the therapy used is effective in controlling T. evansi in cats. (C) 2009 Elsevier B.V. All rights reserved.