Classification of circulating tumor cells by epithelial-mesenchymal transition markers.
Classification of circulating tumor cells by epithelial-mesenchymal transition markers.
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DOI：
10.1371/journal.pone.0123976
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Xu J
中科院分区：
文献类型：
--
作者：
Wu S;Liu S;Liu Z;Huang J;Pu X;Li J;Yang D;Deng H;Yang N;Xu J
在癌症中，上皮间质转化（EMT）与转移相关。表征循环肿瘤细胞 (CTC) 中的 EMT 表型一直具有挑战性，因为基于上皮标记的方法通常用于从血液样本中分离和检测 CTC。本研究的目的是利用优化的 CanPatrol CTC 富集技术，利用 EMT 标记对不同类型癌症的 CTC 进行分类。该技术的第一步是通过基于过滤器的方法分离 CTC；然后，采用基于分支DNA信号放大技术的RNA原位杂交（RNA-ISH）方法根据EMT标记对CTC进行分类。我们的结果表明，采用该技术的肿瘤细胞恢复效率至少为 80%。与未优化的方法相比，新方法灵敏度更高，5 ml 血样中检出的 CTC 数量更多。为了进一步验证新方法，收集了来自肝癌、鼻咽癌、乳腺癌、结肠癌、胃癌或非小细胞肺癌 (NSCLC) 患者的 164 份血液样本，用于 CTC 分离和表征。在 164 份血液样本中的 107 份（65%）中检测到 CTC，并使用 EMT 标记物鉴定出 3 个 CTC 亚群，包括上皮 CTC、生物表型上皮/间质 CTC 和间质 CTC。与癌症早期阶段相比，间充质 CTC 在不同类型癌症的疾病转移阶段的患者中更常见。在癌症转移阶段也检测到具有间充质表型的循环肿瘤微栓子（CTM）。通过 EMT 标志物对 CTC 进行分类有助于识别更具侵袭性的 CTC 亚群，并为确定适当的临床方法提供有用的证据。该方法适用于多种癌症。
In cancer, epithelial-mesenchymal transition (EMT) is associated with metastasis. Characterizing EMT phenotypes in circulating tumor cells (CTCs) has been challenging because epithelial marker-based methods have typically been used for the isolation and detection of CTCs from blood samples. The aim of this study was to use the optimized CanPatrol CTC enrichment technique to classify CTCs using EMT markers in different types of cancers. The first step of this technique was to isolate CTCs via a filter-based method; then, an RNA in situ hybridization (RNA-ISH) method based on the branched DNA signal amplification technology was used to classify the CTCs according to EMT markers. Our results indicated that the efficiency of tumor cell recovery with this technique was at least 80%. When compared with the non-optimized method, the new method was more sensitive and more CTCs were detected in the 5-ml blood samples. To further validate the new method, 164 blood samples from patients with liver, nasopharyngeal, breast, colon, gastric cancer, or non-small-cell lung cancer (NSCLC) were collected for CTC isolation and characterization. CTCs were detected in 107(65%) of 164 blood samples, and three CTC subpopulations were identified using EMT markers, including epithelial CTCs, biophenotypic epithelial/mesenchymal CTCs, and mesenchymal CTCs. Compared with the earlier stages of cancer, mesenchymal CTCs were more commonly found in patients in the metastatic stages of the disease in different types of cancers. Circulating tumor microemboli (CTM) with a mesenchymal phenotype were also detected in the metastatic stages of cancer. Classifying CTCs by EMT markers helps to identify the more aggressive CTC subpopulation and provides useful evidence for determining an appropriate clinical approach. This method is suitable for a broad range of carcinomas.
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DOI：
10.1016/j.biocel.2007.07.011
发表时间：
2007-01-01
影响因子：
4
作者：
Guarino, Marcello
通讯作者：
Guarino, Marcello
DOI：
10.1186/bcr2907
发表时间：
2011-06-15
期刊：
Breast cancer research : BCR
影响因子：
--
作者：
Giuliano M;Giordano A;Jackson S;Hess KR;De Giorgi U;Mego M;Handy BC;Ueno NT;Alvarez RH;De Laurentiis M;De Placido S;Valero V;Hortobagyi GN;Reuben JM;Cristofanilli M
通讯作者：
Cristofanilli M
影响因子：
64.5
作者：
Yang, J;Mani, SA;Weinberg, RA
通讯作者：
Weinberg, RA
影响因子：
3.8
作者：
Gorges TM;Tinhofer I;Drosch M;Röse L;Zollner TM;Krahn T;von Ahsen O
通讯作者：
von Ahsen O
影响因子：
2.5
作者：
Yu, Wenli;Kamara, Harold;Svoboda, Kathy K. H.
通讯作者：
Svoboda, Kathy K. H.