Melanoma growth stimulatory activity enhances the phosphorylation of the class II interleukin-8 receptor in non-hematopoietic cells.
Melanoma growth stimulatory activity enhances the phosphorylation of the class II interleukin-8 receptor in non-hematopoietic cells.
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DOI：
10.1016/s0021-9258(17)42123-5
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发表时间：
1994-01
期刊：
影响因子：
--
通讯作者：
S. Mueller;W. Schraw;A. Richmond
中科院分区：
文献类型：
--
作者：
S. Mueller;W. Schraw;A. Richmond
II类IL-8受体（IL-8 R）以高亲和力结合黑素瘤生长刺激活性（MGSA）和IL-8。逆转录聚合酶链反应表明，II类IL-8 R mRNA，这以前只在造血谱系细胞中检测到，也表达在非造血细胞类型显示MGSA或IL-8响应。为了研究MGSA通过非造血细胞中的II类IL-8 R的信号传导机制，该受体在3ASubE人胎盘和293人肾细胞系中过表达。表达II类IL-8 R的3ASubE转染子的膜制备物表现出响应MGSA处理（0.2 μ M）的GTP γ 35 S结合的2.3 +/-0.2倍增加，其对百日咳毒素敏感。在用亲本表达载体转染的细胞中未观察到这种MGSA应答。体内磷酸化研究表明，II类IL-8 R在未处理的转染子中基本磷酸化，并且MGSA（5 nM）处理显著增强该受体的磷酸化。MGSA诱导的受体磷酸化是时间和浓度依赖性的，并且可以通过用钙离子载体A23187处理来模拟。磷酸氨基酸分析表明，MGSA诱导的受体磷酸化是在丝氨酸残基上，表明丝氨酸激酶响应于MGSA与非造血细胞中的II类IL-8 R结合而被激活。
The class II IL-8 receptor (IL-8R) binds both melanoma growth stimulatory activity (MGSA) and IL-8 with high affinity. Reverse transcriptase polymerase chain reaction demonstrates that the class II IL-8R mRNA, which has previously been detected only in cells of hematopoietic lineage, is also expressed in non-hematopoietic cell types shown to respond to MGSA or IL-8. To study the signaling mechanism by MGSA through the class II IL-8R in non-hematopoietic cells, this receptor was overexpressed in the 3ASubE human placental and the 293 human kidney cell lines. Membrane preparations of the class II IL-8R expressing 3ASubE transfectants exhibited a 2.3 +/- 0.2-fold increase in GTP gamma 35S binding, which was sensitive to pertussis toxin, in response to MGSA treatment (0.2 microM). This MGSA response was not observed in cells transfected with the parental expression vector. In vivo phosphorylation studies demonstrated that the class II IL-8R was basally phosphorylated in the untreated transfectants, and MGSA (5 nM) treatment markedly enhanced the phosphorylation of this receptor. The MGSA-induced receptor phosphorylation was both time and concentration dependent and could be mimicked by treatment with the calcium ionophore A23187. Phosphoamino acid analysis indicated that the MGSA-induced receptor phosphorylation was on serine residue(s), suggesting that a serine kinase is activated in response to MGSA binding to the class II IL-8R in non-hematopoietic cells.