Infectivity of asymptomatic Plasmodium-infected human populations to Anopheles dirus mosquitoes in western Thailand
Infectivity of asymptomatic Plasmodium-infected human populations to Anopheles dirus mosquitoes in western Thailand
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DOI：
10.1603/0022-2585-41.2.201
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发表时间：
2004-03-01
影响因子：
2.1
通讯作者：
Sattabongkot, JN
中科院分区：
文献类型：
--
作者：
Coleman, RE;Kumpitak, C;Sattabongkot, JN
在泰国西部，疟原虫感染者的传染性通过喂食实验室饲养的大劣按蚊佩顿和哈里森蚊，将静脉血油置于膜喂食装置中进行评估。在2000年5月至2001年11月期间，通过显微镜检查了在一项积极的疟疾监测计划期间收集的总共6，494张血膜，以确定是否存在疟原虫寄生虫：3.3%、4.5%和0.1%的载玻片为恶性疟原虫（Pf）、间日疟原虫（Pv）和三日疟原虫（Pm）阳性。分别从Pf、Pv、Pm和Pf/Pv混合感染的70、52、6和4个个体采集静脉血，167个未感染个体作为阴性对照。用Pf-、Pv-和Pm-感染的血液进行的膜喂养仅10%（7/70）、13%（7/52）和0%（0/6）产生感染的蚊子。百分之一（2/167）的显微镜阴性样品感染了蚊子;然而。随后通过聚合酶链反应确定两个样品均为Pf阳性。仅在29%（4/14）的感染样本中观察到配子细胞。所有感染导致低卵囊负荷（平均1.2个卵囊/阳性蚊子）。7个感染性Pf样品中只有4.5%（10/222）的蚊子产生了卵囊，而7个感染性Pv样品中有2.9%（9/311）的蚊子产生了卵囊。据估计，蚊子在Kong Mon-Tha的人口中吸血后感染Pf或Pv的可能性分别为6，700分之一和5，700分之一。对泰国西部疟疾传播的影响进行了讨论。
The infectivity of Plasmodium-infected humans in western Thailand was estimated by feeding laboratory-reared Anopheles dirus Peyton and Harrison mosquitoes oil venous blood placed in a membrane-feeding apparatus, Between May 2000 and November 2001, a total of 6,494 blood films collected during an active malaria surveillance program were checked by microscopy For the presence of Plasmodium parasites: 3.3, 4.5, and 0.1% of slides were P. falciparum- (Pf), P. vivax- (Pv), and P. malariae (Pm)-positive. Venous blood was collected from 70, 52, 6, and 4 individuals infected with Pf, Pv, Pm, and mixed Pf/Pv, respectively, with 167 uninfected individuals serving as negative controls. Only 10% (7/70), 13% (7/52), and 0% (0/6) of membrane feeds conducted on Pf-, Pv-, and Pm-infected blood yielded infected mosquitoes. One percent (2/167) of microscope-negative samples infected mosquitoes; however. both samples were subsequently determined to be Pf-positive by polymerase chain reaction. Gametocytes were observed in only 29% (4/14) of the infectious samples. All infectious resulted in low oocyst loads (average of 1.2 oocysts per positive mosquito). Only 4.5% (10/222) of mosquitoes fed on I the seven infectious Pf samples developed oocysts, whereas 2.9% (9/311) of mosquitoes fed on the seven infectious Pv samples developed oocysts. The probability of a mosquito becoming infected with Pf or Pv after a blood meal on a member of the human population in Kong Mon-Tha was estimated to be I in 6,700 and I in 5,700, respectively. The implications toward malaria transmission in western Thailand are discussed.