Kontinuierliche Reaktionsführung mit löslichen Enzymen
Kontinuierliche Reaktionsführung mit löslichen Enzymen
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与酶相关的连续反应
DOI：
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发表时间：
1992
期刊：
影响因子：
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通讯作者：
C. Wandrey
中科院分区：
文献类型：
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作者：
U. Kragl;Durda Vasic;C. Wandrey
可溶酶的连续处理。本文研究了连续操作的强制流动酶膜反应器的使用，其中可溶性酶在反应容器中的保留是通过超滤膜实现的。该技术已在乙酰化酶法合成l -氨基酸的200吨/年水平上实现商业化。这对于具有辅助因子再生的多酶体系的应用尤其有用。从相应的α-酮酸合成l -叔亮氨酸已达到公斤级。与水溶性聚合物偶联的辅酶与酶一起保留在膜反应器中。使用合适的条件防止酶和辅酶通过膜或失活的损失。因此，酶和辅酶的成本不再是经济过程的限制。在连续运行的酶膜反应器中，辅酶的再生次数可达60万次。在连续多肽合成中，时空产率可达25 kg/(l d)。为了抑制副反应，可以使用非常高的催化剂浓度，使停留时间低于4分钟。
Continuous processes with soluble enzymes. This paper surveys the use of continuously operating enzyme-membrane reactors with enforced flow where the retention of soluble enzymes in the reaction vessel is achieved by means of an ultrafiltration membrane. This technique has been commercialized in the acylase process for the synthesis of L-amino acids on a 200 ton/year level. It is especially useful for the application of multi-enzyme systems with cofactor regeneration. The synthesis of L-tert-leucine from the corresponding α-keto acid has been achieved on a kilogram scale. Coenzymes coupled to water soluble polymers are retained in the membrane-reactor together with the enzymes. Use of suitable conditions prevents loss of enzyme and coenzyme by passage through the membrane or by deactivation. Therefore the costs of enzymes and coenzymes are no longer limitations for economic processes. In the continuously operating enzyme-membrane reactor regeneration of the coenzyme up to 600 000 times was achieved. In continuous peptide synthesis space-time yields of 25 kg/(l d) were obtained. To suppress side reactions very high catalyst concentrations are possible, yielding residence times below 4 min.