Increased plasma glutamate in non-smokers with vasospastic angina pectoris is associated with plasma cystine and antioxidant capacity
Increased plasma glutamate in non-smokers with vasospastic angina pectoris is associated with plasma cystine and antioxidant capacity
复制标题
患有血管痉挛性心绞痛的非吸烟者血浆谷氨酸含量增加与血浆胱氨酸和抗氧化能力相关
DOI：
10.1080/14017431.2022.2085884
复制
发表时间：
2022
影响因子：
2.2
通讯作者：
Kajinami K
中科院分区：
文献类型：
--
作者：
Oda M;Fujibayashi K;Wakasa M;Takano S;Fujita W;Kitayama M;Nakanishi H;Saito K;Kawai Y;Kajinami K
目标.氧化应激引起的内皮功能障碍在血管痉挛性心绞痛（VSAP）的发生发展中起重要作用。谷氨酸通过产生氧化应激引起内皮功能障碍，并且其通过胱氨酸/谷氨酸反向转运蛋白（XC-）抑制胱氨酸输入内皮细胞，这导致抗氧化剂谷胱甘肽的耗尽。然而，谷氨酸和胱氨酸是否与VSAP的发病机制有关仍不清楚。我们研究了非吸烟VSAP患者的血浆谷氨酸和胱氨酸水平、氧化应激标志物和抗氧化能力，以确定谷氨酸和胱氨酸是否与VSAP的发生相关。我们评估了49名非吸烟者，将其分为VSAP组（n= 27）和非VSAP组（n= 22），并测定了血浆谷氨酸、胱氨酸、硝基酪氨酸、活性氧代谢产物和生物抗氧化潜力。VSAP组的血浆谷氨酸和胱氨酸值显著高于无VSAP组（分别为59.8 ± 25.7 vs. 43.5 ± 18.7 µmol/L，p= 0.016和35.3 ± 14.2 vs. 25.2 ± 9.1 µmol/L，p= 0.0056）。血浆谷氨酸和胱氨酸值显著正相关（r= 0.32，p = 0.027）。氧化应激标志物硝基酪氨酸和活性氧代谢产物的水平，以及作为衡量抗氧化能力的生物抗氧化潜力，在两组之间没有显着差异。然而，谷氨酸和生物抗氧化潜力值呈显著负相关（r=-0.3，p = 0.036）。不吸烟的VSAP患者血浆谷氨酸水平升高，与血浆胱氨酸呈正相关，与生物抗氧化潜力水平呈负相关。
Objectives. Endothelial dysfunction caused by oxidative stress plays an important role in the development of vasospastic angina pectoris (VSAP). Glutamate causes endothelial dysfunction by generating oxidative stress, and it inhibits cystine import into endothelial cells via the cystine/glutamate antiporter (XC–), which leads to depletion of antioxidant glutathione. However, whether glutamate and cystine are implicated in the pathogenesis of VSAP remains unclear. We investigated plasma glutamate and cystine levels, oxidative stress markers and antioxidant capacity in non-smoker patients with VSAP to determine whether glutamate and cystine are associated with the development of VSAP. We assessed 49 non-smokers assigned to groups with (n= 27) and without (n= 22) VSAP, and also measured plasma glutamate, cystine, nitrotyrosine, reactive oxygen metabolites and biological antioxidant potential.Results. Plasma glutamate and cystine values were significantly higher in the group with, than without VSAP (59.8 ± 25.7 vs. 43.5 ± 18.7 µmol/L,p= .016 and 35.3 ± 14.2 vs. 25.2 ± 9.1 µmol/L,p= .0056, respectively). Plasma glutamate and cystine values were significantly and positively associated (r= 0.32,p= .027). Levels of the oxidative stress markers nitrotyrosine and reactive oxygen metabolites, and biological antioxidant potential of as a measure of antioxidant capacity, did not significantly differ between the two groups. However, glutamate and biological antioxidant potential values were significantly and negatively associated (r= −0.3,p= .036).Conclusion. Plasma glutamate levels were increased in patients with VSAP who did not smoke, and they were positively associated with plasma cystine and negatively associated with the biological antioxidant potential levels.