PRAZIQUANTEL-INDUCED EXPOSURE OF SCHISTOSOMA-MANSONI ALKALINE-PHOSPHATASE - DRUG-ANTIBODY SYNERGY WHICH ACTS PREFERENTIALLY AGAINST FEMALE WORMS
PRAZIQUANTEL-INDUCED EXPOSURE OF SCHISTOSOMA-MANSONI ALKALINE-PHOSPHATASE - DRUG-ANTIBODY SYNERGY WHICH ACTS PREFERENTIALLY AGAINST FEMALE WORMS
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DOI：
10.1111/j.1365-3024.1994.tb00307.x
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发表时间：
1994-10-01
影响因子：
2.2
通讯作者：
DOENHOFF, MJ
中科院分区：
文献类型：
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作者：
FALLON, PG;SMITH, P;DOENHOFF, MJ
同时注射具有寄生虫抗原特异性的兔抗血清，可提高吡喹酮治疗小鼠曼氏血吸虫感染的疗效。抗曼氏血吸虫碱性磷酸酶的单特异性兔血清与药物处理的雌虫表面的碱性磷酸酶反应，PZQ发现显著并优先增加雌虫的死亡率。从抗碱性磷酸酶抗血清中提纯的免疫球蛋白对吡喹酮处理的虫体表面碱性磷酸酶活力有54%的抑制作用。我们认为，协同抗体介导的药物损伤的蠕虫死亡是被动转移的抗体抑制药物暴露的雌性蠕虫表面碱性磷酸酶的结果。
The efficacy of praziquantel-treatment of murine Schistosoma mansoni-infections can be enhanced by concurrent administration of rabbit anti-sera with specificity for parasite antigens. Monospecific rabbit serum raised against S. mansoni worm alkaline phosphatase, that was reactive with the enzyme an the drug-treated female surface, was found to significantly and preferentially increase the mortality of female worms by PZQ. Immunoglobulins purified from the anti-alkaline phosphatase antiserum inhibited 54% of schistosome alkaline phosphatase enzymatic activity on the surface of praziquantel-treated worms. We propose that synergistic antibody-mediated death of drug-damaged worms is a consequence of the inhibition of drug-exposed alkaline phosphatase on the female worm surface by passively transferred antibody.