eIF4A2 is a host factor required for efficient HIV-1 replication
eIF4A2 is a host factor required for efficient HIV-1 replication
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DOI：
10.1016/j.micinf.2018.05.001
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发表时间：
2018-06-01
影响因子：
5.8
通讯作者：
Yamaoka, Shoji
中科院分区：
文献类型：
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作者：
Ndzinu, Jerry Kwame;Takeuchi, Hiroaki;Yamaoka, Shoji
宿主因子是HIV-1有效复制所必需的。为了鉴定这些因子，使用人T细胞系进行全基因组RNA干扰筛选。在本研究中，我们评估是否真核翻译起始因子4A亚型2（eIF 4A 2），在我们的屏幕上确定的DEAD盒蛋白，是必要的有效的HIV-1复制。通过使用慢病毒系统稳定表达eIF 4A 2特异性短发夹RNA（shRNA），利用eIF 4A 2耗尽的MT4 C5细胞，我们发现eIF 4A 2的耗尽显著抑制了复制型报告基因HIV-1的感染。eIF 4A 2消耗降低了病毒cDNA合成的效率，病毒体进入靶细胞不受影响。eIF 4A 2的缺失也以敲低水平依赖性方式抑制HIV-1传播感染。这些结果表明，HIV-1需要eIF 4A 2在人T细胞中进行最佳复制。(C)2018作者由Elsevier Masson SAS代表巴斯德研究所发布。
Host factors are required for efficient HIV-1 replication. To identify these factors, genome-wide RNA interference screening was performed using a human T cell line. In the present study, we assessed whether eukaryotic translation initiation factor 4A isoform 2 (eIF4A2), a DEAD-box protein identified in our screen, is necessary for efficient HIV-1 replication. Exploiting MT4C5 cells depleted of eIF4A2 by stable expression of eIF4A2-specific short-hairpin RNA (shRNA) using a lentiviral system, we found that depletion of eIF4A2 markedly inhibited the infection of a replication-competent reporter HIV-1. eIF4A2 depletion reduced the efficiency of viral cDNA synthesis with virion entry into target cells being unaffected. Depletion of eIF4A2 also inhibited HIV-1 spreading infection in a knockdown level-dependent manner. These results suggest that HIV-1 requires eIF4A2 for optimal replication in human T cells. (C) 2018 The Authors. Published by Elsevier Masson SAS on behalf of Institut Pasteur.