Identification of candidate genes involved in somatosensory functions of cranial sensory ganglia
Identification of candidate genes involved in somatosensory functions of cranial sensory ganglia
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DOI：
10.1016/j.molbrainres.2004.03.024
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发表时间：
2004-07-05
期刊：
影响因子：
--
通讯作者：
Abe, K
中科院分区：
文献类型：
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作者：
Matsumoto, I;Nagamatsu, N;Abe, K
我们已经表明，通过DNA微阵列数据的层次聚类分析获得的组织树的特征表明基因簇中基因的细胞表达模式[J. Neurosci.第74（2003）818号决议]。我们在这里确定了三个基因簇，包含11个基因，作为一个潜在的候选基因库，与颅结构，如面部，口腔和咽部的躯体感觉。为了获得细胞表达谱，对除了先前分析的三个基因之外的八个基因进行原位杂交分析。结果表明，11个阳性细胞分布图基本一致，提示阳性细胞可能是三叉神经节和岩神经节的躯体感觉神经元。trkA、NaN和甘丙肽等6个基因的表达谱和可能的生理功能表明它们直接参与特定的躯体感觉功能，如伤害感受。另一个基因calretinin的功能与机械感觉和本体感觉有关。其余四个基因，包括水通道蛋白I和两个EST克隆，在感觉神经元中的作用是未知的，可能为了解TG和PG的感觉功能提供线索。（C）2004 Elsevier B.V.保留所有权利。
We have shown that the characteristics of tissue trees obtained by the hierarchical cluster analysis of DNA microarray data suggest the cellular expression patterns of genes in the gene clusters [J. Neurosci. Res. 74 (2003) 818]. We here identified three gene clusters containing 11 genes as a potential pool of candidate genes related to somatosensation in cranial structures such as the face, oral cavity and pharynx. To obtain the cellular expression profiles, eight genes other than three genes analyzed previously were subjected to in situ hybridization analysis. The results show that all of the 11 profiles are roughly similar and suggest that the positive cells are probably somatosensory neurons in two cranial sensory ganglia, the trigeminal and petrosal ganglia. The expression profiles and probable physiological functions of the 6 genes such as trkA, NaN and galanin suggest their direct involvement in specific somatosensory functions such as nociception. The function of another gene, calretinin, is putatively related to mechanosensation and proprioception. The roles of the remaining four genes, including aquaporin I and two EST clones, in sensory neurons are unknown, and may provide clues to understand the sensory function in TG and PG. (C) 2004 Elsevier B.V. All rights reserved.