Endothelin 1-mediated regulation of pharyngeal bone development in zebrafish
Endothelin 1-mediated regulation of pharyngeal bone development in zebrafish
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DOI：
10.1242/dev.00338
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发表时间：
2003-04-01
期刊：
影响因子：
4.6
通讯作者：
Crump, JG
中科院分区：
文献类型：
--
作者：
Kimmel, CB;Ullmann, B;Crump, JG
内皮素1（Edn 1）是一种分泌肽，在斑马鱼咽弓原基的腹侧表达，是咽软骨发育的正确模式所必需的。我们已经研究了突变体和吗啉注射的幼虫，以研究Edn 1信号在受精后第一周内在前咽弓骨发育中的作用。我们观察到Edn 1减少后前弓真皮骨的各种表型变化，包括丢失、尺寸减小和扩张、融合和形状变化。值得注意的是，发生的变化似乎与残留Edn 1的水平有关。下颌弓真皮骨融合发生严重Edn 1损失。在背侧舌骨弓中，当Edn 1严重减少时，真皮鳃盖骨通常不存在，并且当Edn 1仅轻度减少时，真皮鳃盖骨通常增大，这表明相同的信号可以在控制单个骨的发育中起积极和消极的作用。位置似乎也会影响这些变化：鳃蒂骨条，一个通常在鳃盖骨腹侧的真皮舌骨，在同一个足弓中，在鳃盖骨扩大的地方可能会缺失。我们建议Edn 1作为一种形态发生素;不同水平的图案特定的位置，形状和大小的骨沿着背腹轴。涉及Edn 1的变化可能发生在Actinopterygian进化过程中，以产生硬骨鱼类有效的鳃泵鳃盖装置。
Endothelin 1 (Edn1), a secreted peptide expressed ventrally in the primordia of the zebrafish pharyngeal arches, is required for correct patterning of pharyngeal cartilage development. We have studied mutants and morpholino-injected larvae to examine the role of the Edn1 signal in patterning anterior pharyngeal arch bone development during the first week after fertilization. We observe a remarkable variety of phenotypic changes in dermal bones of the anterior arches after Edn1 reduction, including loss, size reduction and expansion, fusion and shape change. Notably, the changes that occur appear to relate to the level of residual Edn1. Mandibular arch dermal bone fusions occur with severe Edn1 loss. In the dorsal hyoid arch, the dermal opercle bone is usually absent when Edn1 is severely reduced and is usually enlarged when Edn1 is only mildly reduced, suggesting that the same signal can act both positively and negatively in controlling development of a single bone. Position also appears to influence the changes: a branchiostegal ray, a dermal hyoid bone normally ventral to the opercle, can be missing in the same arch where the opercle is enlarged. We propose that Edn1 acts as a morphogen; different levels pattern specific positions, shapes and sizes of bones along the dorso-ventral axis. Changes involving Edn1 may have occurred during actinopterygian evolution to produce the efficient gill-pumping opercular apparatus of teleosts.