MORPHOMETRIC STUDIES ON HAMSTER TESTES IN GONADALLY ACTIVE AND INACTIVE STATES - LIGHT-MICROSCOPE FINDINGS
MORPHOMETRIC STUDIES ON HAMSTER TESTES IN GONADALLY ACTIVE AND INACTIVE STATES - LIGHT-MICROSCOPE FINDINGS
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DOI：
10.1095/biolreprod39.5.1225
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发表时间：
1988-12-01
影响因子：
3.6
通讯作者：
RUSSELL, LD
中科院分区：
文献类型：
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作者：
HIKIM, APS;BARTKE, A;RUSSELL, LD
本研究提供了定量信息的睾丸季节性繁殖金黄仓鼠在活跃和退化状态的性腺激素活性。在成年性腺活跃的动物中，生精小管占睾丸体积的92.5%。间质细胞占睾丸体积的1.4%。单个Leydig细胞的平均体积为1092 μ m3，并且每个睾丸含有约2540万个Leydig细胞。在周期的VII-VIII期期间，平均支持细胞核的体积为502 μ m3。性腺活动活跃状态下的一克仓鼠睾丸含有4450万个支持细胞，整个睾丸含有约7380万个支持细胞。短光照12-13周的仓鼠睾丸体积缩小90%，同时生精小管体积也减少（减少90.8%），管状内腔（98.8%），氚（72.7%），间质细胞室睾丸间质细胞（69.7%）、间质细胞核（50.0%）、血管（85.5%）、巨噬细胞（68.9%）和支持细胞核（34.1%）。曲细精管的直径（61.1%）和长度（36.8%）也减小。虽然在短时间光照后每个睾丸的Leydig细胞数量显著降低（p < 0.02），但每个睾丸的Sertoli细胞数量保持不变。单个支持细胞在性腺活跃的仓鼠容纳，平均而言，2.27前细线期精母细胞，2.46粗线期精母细胞，和8.17圆形精子细胞;相应的数字在退化的睾丸分别为0.96，0.20和0.04，分别。光周期引起的内分泌功能改变，使性腺活动活跃状态和退化状态下的睾丸结构发生显著变化，提示金黄地鼠可作为研究睾丸结构与功能关系的模型。
This study provides quantitative information on the testes of seasonally breeding golden hamsters during active and regressed states of gonadol activity. Seminiferous tubules occupied 92.5% of testis volume in adult gonadally active animals. Leydig cells constituted 1.4% of the testicular volume. The mean volume of an individual Leydig cell was 1092 .mu.m3, and each testis contained about 25.4 million Leydig cells. The volume of an average Sertoli cell nucleus during stage VII-VIII of the cycle was 502 .mu.m3. A gram of hamster testis during the active state of gonadal activity contained 44.5 million Sertoli cells, and the entire testis contained approximately 73.8 million Sertoli cells. Testes of the hamsters exposed to short photoperiods for 12-13 wk displayed a 90% reduction in testis volume that was associated with a decrease in the volume of semiferous tubules (90.8% reduction), tubular lumena (98.8%), interstitium (72.7%), Leydig cell compartment (79.3%), individual Leydig cells (69.7%), Leydig cell nuclei (50.0%), blood vessels (85.5%), macrophages (68.9%), and Sertoli cell nuclei (34.1%). The diameter (61.1%) and the length (36.8%) of the seminiferous tubules were also decreased. Although the number of Leydig cells per testis was significantly lower (p < 0.02) after short-photoperiod exposure, the number of Sertoli cells per testis remained unchanged. The individual Sertoli cell in gonadally active hamsters accommodated, on the average, 2.27 pre-leptotene spermatocytes, 2.46 pachytene spermatocytes, and 8.17 round spermatids; the corresponding numbers in the regressed testes were 0.96, 0.20, and 0.04, respectively. The striking differences in the testicular structure between the active and regressed states of gonadal activity follow photoperiod-induced changes in endocrine function and suggest that the golden hamster may be used as a model to study structure-function relationship in the testis.