Microdialysis separately monitors myocardial interstitial myoglobin during ischemia and reperfusion
Microdialysis separately monitors myocardial interstitial myoglobin during ischemia and reperfusion
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DOI：
10.1152/ajpheart.01207.2004
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发表时间：
2005-08-01
影响因子：
4.8
通讯作者：
Mori, H
中科院分区：
文献类型：
--
作者：
Kitagawa, H;Yamazaki, T;Mori, H
直接监测肌红蛋白外排在缺血和再灌注一直受到限制，因为固有的样本收集问题在缺血区域。最近，心脏透析技术为监测心脏缺血区低分子量化合物的心肌间质水平提供了一种强有力的方法。在本研究中，我们通过使用具有高分子质量切断的微透析探针将分子靶标扩展到高分子量化合物，并监测心肌间质肌红蛋白水平。将透析探针植入麻醉兔左心室游离壁。冠状动脉主干闭塞60或120分钟。我们检测了心肌缺血和再灌注对心肌间质肌红蛋白水平的影响。缺血15 min间质肌红蛋白升高，缺血120 min间质肌红蛋白继续升高，缺血45 min时血肌红蛋白升高。同期间质间隙乳酸和肌红蛋白升高。缺血60分钟时，再灌注显著加速间质肌红蛋白释放。再灌注后0-15分钟间质肌红蛋白水平比冠状动脉闭塞后60-75分钟高5倍。透析技术允许早期检测肌红蛋白释放和单独监测肌红蛋白释放在缺血和再灌注。心肌间质肌红蛋白水平可作为心肌缺血或再灌注损伤的指标。
Direct monitoring of myoglobin efflux during ischemia and reperfusion has been limited because of inherent sample collection problems in the ischemic region. Recently, the cardiac dialysis technique has offered a powerful method for monitoring myocardial interstitial levels of low-molecular-weight compounds in the cardiac ischemic region. In the present study, we extended the molecular target to high-molecular-weight compounds by use of microdialysis probes with a high-molecular-mass cutoff and monitored myocardial interstitial myoglobin levels. A dialysis probe was implanted in the left ventricular free wall in anesthetized rabbits. The main coronary artery was occluded for 60 or 120 min. We examined the effects of myocardial ischemia and reperfusion on myocardial interstitial myoglobin levels. Interstitial myoglobin increased within 15 min of ischemia and continued to increase during 120 min of ischemia, whereas blood myoglobin increased at 45 min of ischemia. Lactate and myoglobin in the interstitial space increased during the same period. At 60 min of ischemia, reperfusion markedly accelerated interstitial myoglobin release. The interstitial myoglobin level was fivefold higher at 0-15 min of reperfusion than at 60-75 min of coronary occlusion. The dialysis technique permits earlier detection of myoglobin release and separately monitors myoglobin release during ischemia and reperfusion. Myocardial interstitial myoglobin levels can serve as an index of myocardial injury evoked by ischemia or reperfusion.