Mapping the microscale origins of magnetic resonance image contrast with subcellular diamond magnetometry.
Mapping the microscale origins of magnetic resonance image contrast with subcellular diamond magnetometry.
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DOI：
10.1038/s41467-017-02471-7
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发表时间：
2018-01-09
影响因子：
16.6
通讯作者：
Shapiro MG
中科院分区：
文献类型：
--
作者：
Davis HC;Ramesh P;Bhatnagar A;Lee-Gosselin A;Barry JF;Glenn DR;Walsworth RL;Shapiro MG
磁共振成像（MRI）是一种广泛使用的生物医学成像模式，其大部分对比度来自组织中的微尺度磁场模式。然而，这些模式和宏观尺度的MR图像的外观之间的连接一直没有直接的实验研究的主题，由于缺乏方法来映射生物样品中的微观领域。在这里，我们光学探测磁场在哺乳动物细胞和组织中的亚微米分辨率和纳特斯拉的灵敏度，使用氮空位金刚石磁强计，并结合联合收割机这些测量模拟核自旋进动预测相应的MRI对比度。我们在巨噬细胞铁摄取的体外模型和肝脏铁过载小鼠模型的组织学样本中证明了该技术的实用性。此外，我们还跟踪了活细胞中磁性颗粒的内吞作用。这种方法弥合了MRI体素与其微观成分之间的根本差距。磁共振成像从局部磁场中获得其对比度，然而这些磁场与宏观对比度之间的联系尚未通过直接实验建立。在这里，Davis等人使用金刚石磁力测量法以亚微米分辨率绘制哺乳动物细胞内的局部磁场，并预测宏观对比度。
Magnetic resonance imaging (MRI) is a widely used biomedical imaging modality that derives much of its contrast from microscale magnetic field patterns in tissues. However, the connection between these patterns and the appearance of macroscale MR images has not been the subject of direct experimental study due to a lack of methods to map microscopic fields in biological samples. Here, we optically probe magnetic fields in mammalian cells and tissues with submicron resolution and nanotesla sensitivity using nitrogen-vacancy diamond magnetometry, and combine these measurements with simulations of nuclear spin precession to predict the corresponding MRI contrast. We demonstrate the utility of this technology in an in vitro model of macrophage iron uptake and histological samples from a mouse model of hepatic iron overload. In addition, we follow magnetic particle endocytosis in live cells. This approach bridges a fundamental gap between an MRI voxel and its microscopic constituents. Magnetic resonance imaging derives its contrast from local magnetic fields, however the connection between these fields and macroscale contrast has not been established through direct experiments. Here, Davis et al. use diamond magnetometry to map local magnetic fields within mammalian cells with sub-micron resolution and predict macroscale contrast.
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影响因子：
3.3
作者：
Bowen, CV;Zhang, XW;Rutt, BK
通讯作者：
Rutt, BK
影响因子：
3.3
作者：
Ghugre, Nilesh R.;Wood, John C.
通讯作者：
Wood, John C.
影响因子：
5.7
作者：
Deans AE;Wadghiri YZ;Aristizábal O;Turnbull DH
通讯作者：
Turnbull DH
DOI：
10.1158/1078-0432.ccr-10-3420
发表时间：
2011-09-01
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Daldrup-Link HE;Golovko D;Ruffell B;Denardo DG;Castaneda R;Ansari C;Rao J;Tikhomirov GA;Wendland MF;Corot C;Coussens LM
通讯作者：
Coussens LM
影响因子：
2.5
作者：
Matsumoto, Yuri;Jasanoff, Alan
通讯作者：
Jasanoff, Alan