Bone formation induced by a novel form of mechanical loading on joint tissue.
Bone formation induced by a novel form of mechanical loading on joint tissue.
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DOI：
10.2187/bss.18.41
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发表时间：
2004-06-01
期刊：
影响因子：
--
通讯作者：
Yokota, Hiroki
中科院分区：
文献类型：
--
作者：
Tanaka, Shigeo M;Sun, Hui B;Yokota, Hiroki
由于机械负荷不足，在太空飞行中暴露于失重状态会降低骨量。为了在失重状态下保持骨量，我们研究了一种新的机械载荷形式--关节载荷。由于重力引起的骨骼系统负荷的一部分被关节组织的粘弹性变形所吸收，我们假设关节组织的变形会在骨中产生流体流动并刺激骨干皮质骨中的骨形成。为了验证这一假设，我们对小鼠肘关节直接施加振荡载荷，并对尺骨骨干进行骨组织形态计量学研究。使用离体鼠股骨，测量流动电位以评价由关节负荷诱导的流体流动。骨组织形态计量学显示，与无负荷对照组相比，肘部负荷增加矿化表面，矿物质沉积率和骨形成率分别为3.2倍，3.0倍和7.9倍。我们证明，关节负荷产生的流动潜力在骨髓腔的股骨。研究结果支持了一种新的机制，其中关节负荷可能通过产生流体流动有效地刺激骨形成，并表明被动或主动驱动的关节支持性附件对于在暴露于失重期间维持宇航员的骨量是有用的。
Because of insufficient mechanical loading, exposure to weightlessness in space flight reduces bone mass. In order to maintain bone mass in a weightless condition, we investigated a novel form of mechanical loading--joint loading. Since some part of gravity-induced loading to our skeletal system is absorbed by viscoelastic deformation of joint tissues, we hypothesized that deformation of joint tissues would generate fluid flow in bone and stimulate bone formation in diaphyseal cortical bone. In order to test the hypothesis, we applied directly oscillatory loading to an elbow joint of mice and conducted bone histomorphometry on the diaphysis of ulnae. Using murine femurs ex vivo, streaming potentials were measured to evaluate a fluid flow induced by joint loading. Bone histomorphometry revealed that compared to no loading control, elbow loading increased mineralizing surface, mineral apposition rate, and bone formation rate 3.2-fold, 3.0-fold, and 7.9-fold, respectively. We demonstrated that joint loading generated a streaming potential in a medullar cavity of femurs. The results support a novel mechanism, in which joint loading stimulates effectively bone formation possibly by generating fluid flow, and suggest that a supportive attachment to joints, driven passively or actively, would be useful to maintain bone mass of astronauts during an exposure to weightlessness.