Fourteenth seminar on trypanosomiasis
Fourteenth seminar on trypanosomiasis
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第十四届锥虫病研讨会
DOI：
10.1016/0035-9203(74)90189-8
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发表时间：
1974
影响因子：
2.2
通讯作者：
W. Kershaw
中科院分区：
文献类型：
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作者：
W. Kershaw
1. 与布氏锥虫和刚果锥虫的血流阶段一样，间日锥虫血流的线粒体基本上是单线，向后扩张形成动质体囊，但与布氏锥虫的线粒体不同，尽管与刚果锥虫相似，间日锥虫的线粒体始终呈心肌黄酶阳性，并具有管状嵴。间日锥虫动体囊的碗状前凹结构是其他唾液锥虫所没有的。2． Swain 菌株的洗涤后的整个有机体制剂以相似的速率氧化葡萄糖、甘油和 1-3 甘油磷酸盐，并以这些底物的大约一半的速率氧化琥珀酸（参见 T. brucei 血流形式从不氧化琥珀酸）。 α-酮戊二酸以非常低（仅可测量）的速率被氧化（参见T. brucei，其中只有短截形式氧化该底物）。所有这些底物的呼吸对氰化物不敏感，但对间氯苯异羟肟酸的抑制100%敏感（0" 1-0" 2 mM m-CLAM 完全消除呼吸）。
1. As in bloodstream stages of T. brucei and T. congolense, the mitochondrion of bloodstream T. vivax is basically a single thread with a posterior expansion to form the kinetoplast capsule, but unlike the mitochondrion of T. brucei, though like that of T. congolense, the mitochondrion of T. vivax is at all times diaphorase-positive and furnished with tubular cristae. The bowl-like anterior concavity of the T. vivax kinetoplast capsule is not found in other salivarian trypanosomes.2. Washed whole organism preparations of the Swain strain oxidize glucose, glycerol and 1-3 glycerophosphate at similar rates and succinate at about half the rate for these substrates (cf. T. brucei bloodstream forms never oxidize succinate). Alpha-oxoglutarate is oxidized at a very low (just measurable) rate (cf. T. brucei where only stumpy forms oxidize this substrate). The respiration of all these substrates is cyanide insensitive but 100% sensitive to inhibition by m-chlorobenzhydroxamic acid (0" 1-0" 2 mM m-CLAM completely abolished respiration).