Cloning and expression of a mouse member of the PLUNC protein family exclusively expressed in tongue epithelium
Cloning and expression of a mouse member of the PLUNC protein family exclusively expressed in tongue epithelium
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DOI：
10.1016/j.ygeno.2003.09.015
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发表时间：
2004-04-01
期刊：
影响因子：
4.4
通讯作者：
Bingle, CD
中科院分区：
文献类型：
--
作者：
LeClair, EE;Nomellini, V;Bingle, CD
腭、肺和鼻上皮克隆（Plunc，现更名为Splunc 1）是在人、小鼠、大鼠和牛的口咽和上气道中表达的小分泌蛋白。这种蛋白质在结构上与已知的宿主防御革兰氏阴性菌的介质同源。我们已经表征了一种新的但与啮齿动物密切相关的基因的基因组序列和表达，我们称之为splunc 5。小鼠Splunc 5序列与小鼠Splunc 1序列60%相同。这些基因还具有高度保守的基因组元件，包括内含子-外显子结构和内含子序列。引人注目的是，splunc 5只在舌背表面的乳头间上皮中表达。通过比较splunc 5，splunc 1，和第三个相关序列，1 plunc 1，在小鼠中的表达谱，我们表明，这些基因表达在独特的结构域沿着一个连续的走廊的口腔，舌，咽，和呼吸上皮。这种表达模式与这些蛋白质保护被潜在病原微生物定殖的上皮表面的假设一致。(C)2003年爱思唯尔公司All rights reserved.
Palate, lung, and nasal epithelium clone (Plunc, now renamed Splunc1) is a small secreted protein expressed in the oropharynx and upper airways of humans, mice, rats, and cows. This protein is structurally homologous to known mediators of host defense against gram-negative bacteria. We have characterized the genomic sequence and expression of a novel but closely related gene from rodents, which we call splunc5. Mouse Splunc5 sequence is 60% identical to mouse Splunc1. The genes also share highly conserved genomic elements including intron-exon structure and intronic sequence. Strikingly, splunc5 is expressed exclusively in the interpapillary epithelium of the tongue's dorsal surface. By comparing the expression profiles of splunc5, splunc1, and a third related sequence, 1plunc1, in mice, we show that these genes are expressed in unique domains along a continuous corridor of oral, lingual, pharyngeal, and respiratory epithelia. This expression pattern is consistent with the hypothesis that these proteins protect epithelial surfaces colonized by potentially pathogenic microorganisms. (C) 2003 Elsevier Inc. All rights reserved.