Characterisation of macrophage migration inhibitory factor from Eimeria species infectious to chickens
Characterisation of macrophage migration inhibitory factor from Eimeria species infectious to chickens
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DOI：
10.1016/j.molbiopara.2006.10.020
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发表时间：
2007-02-01
影响因子：
1.5
通讯作者：
Harper, Susan B.
中科院分区：
文献类型：
--
作者：
Miska, Katarzyna B.;Fetterer, Raymond H.;Harper, Susan B.
巨噬细胞迁移抑制因子 (MIF) 是大约 40 年前被发现的第一个细胞因子。 MIF 的同系物最近从无脊椎动物中分离出来，从进化和功能的角度来看，使其成为一个有趣的分子。本研究是艾美耳属顶复门寄生虫中 MIF 同源物的首次报道。从 Eimeria acervulina 中分离出单个全长克隆，其与脊椎动物的 MIF 具有 35% 至 38% 的氨基酸同一性。来自柔嫩艾美球虫的 MIF cDNA 与弯艾美球虫 MIF 具有 64% 的氨基酸同一性。裂殖子中的 mRNA 表达量最高，而发育中的卵囊和子孢子的表达量低至检测不到的水平。蛋白质表达模式与逆转录酶聚合酶链式反应（RT-PCR）观察到的几乎相同，表明强烈的发育调节。鹿角艾美耳球虫裂殖子的免疫荧光染色和共定位研究表明，MIF 分布在整个细胞质中，并且似乎集中在寄生虫的顶端。在从 E. acervulina 裂殖子收集的排泄/分泌 (ES) 产物中检测到 MIF 的存在，等电聚焦表明此阶段存在三种 MIF 亚型。系统发育分析表明，顶端复合体 MIF 序列与来自拟南芥的 MIF 样分子形成姐妹关系。 (c) 2006 年，Elsevier B.V. 出版
Macrophage migration inhibitory factor (MIF) was the first cytokine to be identified almost 40 years ago. Homologues of MIF have been isolated recently from invertebrates, making it an interesting molecule from an evolutionary as well as functional perspective. The present study represents the first report of MIF homologues in apicomplexan parasites, belonging to the genus Eimeria. A single full-length clone was isolated from Eimeria acervulina that shared between 35 and 38% amino acid identity with MIFs of vertebrates. A MIF cDNA from Eimeria tenella shared 64% amino acid identity with E. acervulina MIF. The mRNA expression was highest in merozoites, whereas developing oocysts and sporozoites expressed low to undetectable levels. Protein expression patterns were nearly identical to that observed by reverse transcriptase polymerase chain reaction (RT-PCR), suggesting strong developmental regulation. Immunofluorescence staining and co-localisation studies of E. acervulina merozoites indicated that MIF is distributed throughout the cytosol, and appears to be concentrated in the apical end of the parasite. The presence of MIF was detected in excretory/secretory (ES) products collected from E. acervulina merozoites, and isoelectric focusing indicated that three MIF isoforms are present in this stage. Phylogenetic analysis revealed that apicomplexan MIF sequences form a sister relationship to MIF-like molecules from Arabidopsis thaliana. (c) 2006 Published by Elsevier B.V.