Molecular genetic analysis of the candidate gene for MOD, a locus required for self-incompatibility in Brassica rapa
Molecular genetic analysis of the candidate gene for MOD, a locus required for self-incompatibility in Brassica rapa
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DOI：
10.1007/s004380000440
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发表时间：
2001-05-01
影响因子：
3.1
通讯作者：
Nasrallah, ME
中科院分区：
文献类型：
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作者：
Fukai, E;Nishio, T;Nasrallah, ME
7MIP-MOD基因编码一种类似水通道蛋白的产物，是甘蓝型油菜自交不亲和反应所必需的MOD基因的候选基因。在一项旨在研究MIP-MOD作用的反义抑制实验中，我们发现14个反义转基因植株以及自交不亲和品种OSome的柱头中MIP-MOD基因的表达水平远远低于自交不亲和的S-8纯合子(S-8)品系的柱头中的水平。因此，我们分析了三个油菜品系：S-8、OSM和自交亲和变种的mip-mod基因的分子结构。黄沙逊(YS)。核苷酸序列分析表明，3株MIP-MOD基因编码相同的氨基酸序列，YS和OSM含有相同的MIP-MOD等位基因，命名为MIP-MODYS。对其他MIP-MODYS等位基因纯合的自交不亲和白菜型油菜品系的分析表明，高水平的MIP-MOD转录本对自交不亲和反应不是必需的。此外，还发现一个由伽马辐射产生的MOD突变体含有野生型MIP-MOD基因，该基因在正常水平下表达。这些数据表明，MIP-MOD本身不是MOD。我们建议将该基因改名为MLM(与MOD连锁的MIP基因)。
7The MIP-MOD (for MOD-locus associated Major Intrinsic Protein) gene encodes an aquaporin-like product, and has been reported to be a candidate for the MOD gene which is required for the self-incompatibility response in Brassica raga. In an antisense suppression experiment designed to investigate the role of MIP-MOD, we found that levels of MIP-MOD mRNA in the stigmas of fourteen antisense transgenics, as well as in the self incompatible cultivar Osome (Osm), were much lower than in the stigmas of the self-incompatible S-8 homozygous (S-8) strain. Therefore, we analyzed the molecular structure of the MIP-MOD gene in three B. rapa strains: S-8, Osm, and the self-compatible var. Yellow Sarson (YS). Nucleotide sequence analysis of the MIP-MOD genes isolated from the three strains revealed that all three encode the same amino acid sequence and that YS and Osm contain the same MIP-MOD allele, designated MIP-MODYS. Analysis of other self-incompatible B. rapa strains that are homozygous for the MIP-MODYS allele indicated that high levels of MIP-MOD transcripts are not essential for the self-incompatibility response. Furthermore, a MOD mutant generated by gamma -irradiation was found to contain a wildtype MIP-MOD gene that is expressed at normal levels. These data suggest that MIP-MOD is not MOD itself. We suggest that this gene should be renamed MLM (for MIP gene linked to MOD).