Homogenous expression of killer cell immunoglobulin-like receptors (KIR) on polyclonal natural killer cells detected by a monoclonal antibody to KIR2D.
Homogenous expression of killer cell immunoglobulin-like receptors (KIR) on polyclonal natural killer cells detected by a monoclonal antibody to KIR2D.
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通过 KIR2D 单克隆抗体检测到多克隆自然杀伤细胞上杀伤细胞免疫球蛋白样受体 (KIR) 的均匀表达。
DOI：
10.1034/j.1399-0039.2000.560306.x
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Long,EO
中科院分区：
文献类型：
--
作者：
Watzl,C;Peterson,M;Long,EO
人自然杀伤（NK）细胞的活性部分受杀伤细胞免疫球蛋白（IG）样受体（KIR）的表达调节，KIR识别I类主要组织相容性复合体（MHC）并可抑制NK细胞的细胞毒性。建立了单克隆抗KIR抗体并命名为Lig 1。Lig 1在细胞表面染色中显示对KIR具有特异性，并且在酶联免疫吸附试验（ELISA）和蛋白质印迹中与所有KIR 2D反应，但不与KIR 3D分子反应，KIR 2DL 4缺乏D1结构域。与其他抗KIR抗体不同，Lig 1不抑制KIR-IG-融合蛋白与MHC-I类表达细胞的结合，也不干扰功能试验中KIR介导的NK细胞细胞毒性抑制。Lig 1与来自不同供体的多克隆NK群体中的所有NK细胞反应，表明所有NK细胞表达至少一种KIR 2D受体。
The activity of human natural killer (NK) cells is in part regulated by the expression of killer cell immunoglobulin (Ig)‐like receptors (KIR) that recognize major histocompatibility complex (MHC) class I and can inhibit NK cell cytotoxicity. A monoclonal anti‐KIR antibody was established and designated Lig1. Lig1 was shown to be specific for KIR in cell‐surface staining and to react with all KIR2D, except KIR2DL4 which lacks a D1 domain, but not with KIR3D molecules in an enzyme‐linked immunoadsorbent assay (ELISA) and Western blotting. Unlike other anti‐KIR antibodies, Lig1 did not inhibit binding of KIR‐Ig‐fusion proteins to MHC‐class I expressing cells nor did it interfere with KIR‐mediated inhibition of NK cell cytotoxicity in a functional assay. Lig1 reacted with all NK cells in polyclonal NK populations from different donors, demonstrating that all NK cells express at least one KIR2D receptor.