S6K1 acts through FOXO to regulate juvenile hormone biosynthesis in the red flour beetle, Tribolium castaneum
S6K1 acts through FOXO to regulate juvenile hormone biosynthesis in the red flour beetle, Tribolium castaneum
复制标题
S6K1 通过 FOXO 调节赤拟谷盗的保幼激素生物合成
DOI：
10.1016/j.jinsphys.2022.104405
复制
发表时间：
2022-06-10
影响因子：
2.2
通讯作者：
Wang, Jianjun
中科院分区：
文献类型：
--
作者：
Jiang, Heng;Zhang, Nan;Wang, Jianjun
核糖体蛋白S6激酶(S6K)作为雷帕霉素复合体1(TORC1)信号通路靶标的下游效应蛋白，是昆虫生殖的重要调节因子，但其作用机制尚不清楚。本研究从赤粉甲中分离到一个S6K基因，命名为TcS6K1。时间和空间表达模式分析表明，TcS6K1在1日龄一龄幼虫和7日龄雌成虫头部中表达水平最高。RNAi介导的TcS6K1在雌性或雄性成虫中的敲除减少了产卵量，伴随而来的是卵黄蛋白原基因TcVg1和TcVg2，两种雄性附腺分泌蛋白，以及与保幼激素(JH)生物合成相关的基因法尼醇脱氢酶(TcFDH)的mRNA水平降低。而转录因子叉头盒O(TcFOXO)的mRNA和蛋白水平没有受到影响，抑制TcS6K1的表达促进了TcFOXO的核转位，从而发挥其转录作用。进一步的RNAi和EMSA分析表明，TcFOXO负调控TcFDH的表达。这些结果表明，S6K可能通过FOXO/JH信号通路调节甲虫的生殖。
As the downstream effector of the target of rapamycin complex 1 (TORC1) signaling pathway, the ribosomal protein S6 kinase (S6K) is an important regulator of insect reproduction, however, the underlying mechanism remains obscure. In this study, a S6K gene, named TcS6K1, was isolated from the red flour beetle, Tribolium castaneum. Analysis of temporal and spatial expression patterns revealed that TcS6K1 is expressed at the highest level in the one-day-old first instar larvae and head of 7-day-old females, respectively. RNAi-mediated knockdown of TcS6K1 in either female or male adults decreased the number of eggs laid, with a concomitant reduction of mRNA levels of vitellogenin genes, TcVg1 and TcVg2, two male accessory gland secretory proteins, as well as the juvenile hormone (JH) biosynthesis-related gene, farnesol dehydrogenase (TcFDH). While the mRNA and protein levels of the transcription factor forkhead box O (TcFOXO) were not affected, suppression of TcS6K1 expression promoted TcFOXO nuclear translocation to exert its transcriptional action. Further RNAi and EMSA analysis revealed that TcFOXO negatively regulated the expression of TcFDH. These results indicate that S6K might regulate beetles' reproduction through FOXO/JH signaling pathway.