The combined effect of electrical stimulation and resistance isometric contraction on muscle atrophy in rat tibialis anterior muscle.
The combined effect of electrical stimulation and resistance isometric contraction on muscle atrophy in rat tibialis anterior muscle.
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DOI：
10.17305/bjbms.2011.2584
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发表时间：
2011-05
影响因子：
3.4
通讯作者：
N. Fujita;S. Murakami;Takamitsu Arakawa;A. Miki;H. Fujino
中科院分区：
文献类型：
--
作者：
N. Fujita;S. Murakami;Takamitsu Arakawa;A. Miki;H. Fujino
电刺激已被用于预防肌肉萎缩，但这种方法在许多以前的研究中是不同的，合适的刺激方案仍然没有确定。虽然阻力运动也被证明是一种有效的对策肌肉萎缩，几乎以前的研究进行了电刺激无阻力。假设没有阻力的电刺激不足以有力地收缩骨骼肌，并且电刺激和有力的阻力收缩的组合比没有阻力的电刺激更有效地减弱肌肉萎缩。本研究探讨了电刺激和阻力性等长收缩对大鼠胫骨前肌萎缩的联合作用。将动物分为对照组、后肢去负荷组（HU）、后肢去负荷+电刺激组（ES）和后肢去负荷+电刺激+电阻性等长收缩组（ES+IC）。电刺激施加到胫骨前肌percent，每天共240秒。ES+IC组，固定踝关节，使其在电刺激时产生阻力性等长收缩。7天后，HU组中各肌纤维类型的横截面积减少。ES+IC组比ES组预防了这些情况。ES组和ES+IC组热休克蛋白72的表达增强。这些结果表明，虽然电刺激是有效的，以防止肌肉萎缩，电刺激和等长收缩的组合有进一步的效果。
Electrical stimulation has been used to prevent muscle atrophy, but this method is different in many previous studies, appropriate stimulation protocol is still not decided. Although resistance exercise has also been shown to be an effective countermeasure on muscle atrophy, almost previous studies carried out an electrical stimulation without resistance. It was hypothesized that electrical stimulation without resistance is insufficient to contract skeletal muscle forcefully, and the combination of electrical stimulation and forceful resistance contraction is more effective than electrical stimulation without resistance to attenuate muscle atrophy. This study investigated the combined effects of electrical stimulation and resistance isometric contraction on muscle atrophy in the rat tibialis anterior muscle. The animals were divided into control, hindlimb unloading (HU), hindlimb unloading plus electrical stimulation (ES), and hindlimb unloading plus the combination of electrical stimulation and resistance isometric contraction (ES+IC). Electrical stimulation was applied to the tibialis anterior muscle percutaneously for total 240 sec per day. In the ES+IC group, the ankle joint was fixed to produce resistance isometric contraction during electrical stimulation. After 7 days, the cross-sectional areas of each muscle fiber type in the HU group decreased. Those were prevented in the ES+IC group rather than the ES group. The expression of heat shock protein 72 was enhanced in the ES and ES+IC groups. These results indicated that although electrical stimulation is effective to prevent muscle atrophy, the combination of electrical stimulation and isometric contraction have further effect.