Effects of exogenous calcium and calcium chelant on cold tolerance of postharvest loquat fruit
Effects of exogenous calcium and calcium chelant on cold tolerance of postharvest loquat fruit
复制标题
外源钙及钙螯合剂对采后枇杷果实耐寒性的影响
DOI：
10.1016/j.scienta.2020.109391
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发表时间：
2020-07-27
影响因子：
4.3
通讯作者：
Jin, Peng
中科院分区：
文献类型：
--
作者：
Li, Ziying;Wang, Li;Jin, Peng
研究了氯化钙（CaCl 2）和钙螯合剂乙二醇双（2-氨基乙基醚）四乙酸（EGTA）对枇杷果实采后冷害的影响。CV. '长红'）在冷藏过程中的变化。结果表明，CaCl 2处理能显著抑制CI的发生，并能维持膜的完整性，降低膜离子渗漏率和丙二醛（MDA）含量。外源钙提高了能荷（EC）和三磷酸腺苷（ATP）、二磷酸腺苷（ADP）含量。同时，CaCl 2处理有效地提高了与能量代谢相关的酶活性，包括H-三磷酸腺苷酶（H+ -ATPaese）、Ca 2 +-三磷酸腺苷酶（Ca 2 +-APTase）、琥珀酸脱氢酶（SDH）、细胞色素c氧化酶（CCO）。CaCl 2处理还提高了δ（1）-吡咯啉-5-羧酸合成酶（P5 CS）、鸟氨酸氨基转移酶（OAT）、谷氨酸脱羧酶（GAD）、二胺氧化酶（DAO）和多胺氧化酶（PAO）的活性，促进了渗透调节物质γ-氨基丁酸（GABA）、脯氨酸和多胺（PAs）的积累。EGTA处理显著提高了CI指数，增加了MDA含量和离子渗漏。EGTA处理的枇杷果实EC和GABA含量也较低。这些结果表明，外源钙能有效提高枇杷果实的耐冷性，并促进其内源渗透调节物质脯氨酸、GABA和PAs的积累。
The effect of calcium chloride (CaCl2) and calcium chelant-ethylene glycol bis (2-aminoethyl ether) tetraacetic acid (EGTA) on chilling injury (CI) of postharvest loquat fruit (Eriobotrya japonica L. cv. 'Changhong') during cold storage was investigated. The results showed that CaCl2 treatment significantly prevented CI and maintained the membrane integrity resulting in the reduction of ion leakage and malondialdehyde (MDA) content. The exogenous calcium increased energy charge (EC) and the content of adenosine triphosphate (ATP), adenosine diphosphate (ADP). Meanwhile, CaCl2 treatment effectively enhanced the enzyme activities associated with energy metabolism including H--adenosine triphosphatase (H+ -ATPaese), Ca2+-adenosine triphosphatase (Ca2+-APTase), succinic dehydrogenase (SDH), cytochrome c oxidase (CCO). Furthermore, the activities of Delta(1)-pyrroline-5-carboxylic acid synthase (P5CS), ornithin-(sic)-aminotransferase (OAT), glutamic acid decarboxylase (GAD), diamine oxidase (DAO) and polyamine oxidase (PAO) were enhanced by CaCl2 treatment, which contributed to the accumulation of osmotic substances gamma-aminobutyric acid (GABA), proline and polyamines (PAs). However, EGTA treatment significantly promoted CI index, increased MDA content and ion leakage. Moreover, low level of EC and GABA were also found in EGTA-treated loquat fruit. These results suggested that exogenous calcium could effectively enhance the chilling tolerance due to the high energy level and promoted accumulation of endogenous osmotic substances proline, GABA and PAs in loquat fruit.