Epidemics and aetiology of hand, foot and mouth disease in Xiamen, China, from 2008 to 2015
Epidemics and aetiology of hand, foot and mouth disease in Xiamen, China, from 2008 to 2015
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DOI：
10.1017/s0950268817000309
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发表时间：
2017-07-01
影响因子：
4.2
通讯作者：
Xia, N. S.
中科院分区：
文献类型：
--
作者：
He, S. Z.;Chen, M. Y.;Xia, N. S.
在过去的8年中，厦门市共发生27227例手足口病病例，其中重症病例99例，死亡6例。本研究旨在探讨厦门市手足口病的分子流行病学特征，为手足口病的诊断方法、疫苗和其他干预措施的发展提供信息。从2009年1月至2015年9月，使用针对HEV 5'非翻译区和病毒蛋白1区的巢式逆转录PCR检测了来自哨点医院的5866份样本。在这些样本中，4290例HEV检测呈阳性，并对扩增子进行测序和基因分型。共鉴定出22种基因型。肠道病毒71型（EV71）和柯萨奇病毒A16、A6和A10型（CA16、CA6和CA10）是最常见的基因型，这些基因型的优势谱系没有变化。EV71每2年成为最主要的基因型。从2013年起，CA6取代CA16成为两种最常见的基因型之一。结果表明，手足口病病原体的多样性，以及次要基因型能够取代主要基因型。我们建议对手足口病病原体的全谱进行长期监测，这可以促进流行预测和诊断检测和疫苗的开发。
Over the past 8 years, human enteroviruses (HEVs) have caused 27 227 cases of hand, foot and mouth disease (HFMD) in Xiamen, including 99 severe cases and six deaths. We aimed to explore the molecular epidemiology of HFMD in Xiamen to inform the development of diagnostic assays, vaccines and other interventions. From January 2009 to September 2015, 5866 samples from sentinel hospitals were tested using nested reverse transcription PCR that targeted the HEV 5' untranslated region and viral protein 1 region. Of these samples, 4290 were tested positive for HEV and the amplicons were sequenced and genotyped. Twenty-two genotypes were identified. Enterovirus 71 (EV71) and coxsackieviruses A16, A6 and A10 (CA16, CA6 and CA10) were the most common genotypes, and there were no changes in the predominant lineages of these genotypes. EV71 became the most predominant genotype every 2 years. From 2013, CA6 replaced CA16 as one of the two most common genotypes. The results demonstrate the vast diversity of HFMD pathogens, and that minor genotypes are able to replace major genotypes. We recommend carrying-out long-term monitoring of the full spectrum of HFMD pathogens, which could facilitate epidemic prediction and the development of diagnostic assays and vaccines.