HISTOLOGICAL, HISTOCYTOCHEMICAL AND ULTRASTRUCTURAL-STUDY ON THE EFFECTS OF SURFACE-CHARGE ON BONE-FORMATION IN THE RABBIT MANDIBLE
HISTOLOGICAL, HISTOCYTOCHEMICAL AND ULTRASTRUCTURAL-STUDY ON THE EFFECTS OF SURFACE-CHARGE ON BONE-FORMATION IN THE RABBIT MANDIBLE
复制标题
DOI：
10.1016/0003-9969(94)00153-3
复制
发表时间：
1995-02-01
影响因子：
3
通讯作者：
OZAWA, H
中科院分区：
文献类型：
--
作者：
HAMAMOTO, N;HAMAMOTO, Y;OZAWA, H
通过在下颌骨颊侧骨膜下植入不带电荷、带负电荷和带正电荷的葡聚糖凝胶珠，研究了不同表面电荷的成骨潜力。在1、2和4周后检查植入部位。在2周时，在带正电荷的珠周围观察到新骨形成。在珠周围观察到的无定形无胶原蛋白层由阴离子物质组成，并含有针状晶体。抗酒石酸酸性磷酸酶阳性的多核巨细胞，具有典型的破骨细胞样皱褶边缘和清晰区带的精细结构特征，在带正电荷的珠粒表面观察到，成骨细胞与它们并列。4周时骨形成仍在进行。在不带电荷或带负电荷的珠周围，在任何时间都没有观察到新骨形成和破骨细胞样细胞。研究结果表明，种植体材料的表面电荷对骨细胞的分化和随后的成骨细胞的出现导致骨形成有一定的影响。
The osteogenic potential of different surface charges was studied by implanting uncharged, negatively charged and positively charged Sephadex beads subperiosteally on the buccal aspect of the mandible. The implant sites were examined 1, 2, and 4 weeks later. New bone formation was observed around the positively charged beads at 2 weeks. An amorphous collagen-free layer seen around the beads consisted of anionic substances and contained needle-like crystals. Tartrate-resistant acid phosphatase-positive, multinucleated giant cells, which had typical fine structural characteristics of osteoclast-like ruffled borders and a clear zone, were observed on the surface of positively charged beads, with osteoblastic cells juxtaposed to them. Bone formation was still going on at 4 weeks in this group. Around the uncharged or negatively charged beads, new bone formation and osteoclast-like cells were not observed at any time. The findings suggest that the surface charge of implant materials has a definite influence on the differentiation of osteoclastic cells and subsequent occurrence of osteoblastic cells leading to formation of bone.