Location and dynamics of a membrane-bound fluorescent hapten. A spectroscopic study.
Location and dynamics of a membrane-bound fluorescent hapten. A spectroscopic study.
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膜结合荧光半抗原的位置和动态。
DOI：
10.1016/0005-2736(84)90212-8
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Owicki,JC
中科院分区：
文献类型：
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作者：
Stanton,SG;Kantor,AB;Petrossian,A;Owicki,JC
在前面的论文（Petrossian，A.和Owicki，J.C.（1984），Biochim. Biophys. Acta 776，217-227），我们描述了单克隆抗荧光素抗体与膜结合的荧光素-脂质半抗原的结合。这些结果表明，一些半抗原荧光素部分延伸远离膜表面并可用于抗体结合，而一些半抗原被隔离并不能立即用于抗体结合。在本文中，我们进行了光谱研究的膜结合的半抗原，并表明，有一个以上的物理上不同的荧光团环境，与隔离的半抗原与磷脂头基区域。膜相关的荧光团的量取决于膜脂质组合物：当脂质是不饱和或支链磷脂酰胆碱（PC）时，大多数荧光团是相关的，而半抗原在很大程度上扩展为PC/胆固醇混合物。胆固醇对膜结合半抗原与抗体结合的可用性的影响并不是该系统所独有的。隔离的和延伸的半抗原之间的转化是缓慢的（分钟）。
In the preceding paper (Petrossian, A. and Owicki, J.C. (1984), Biochim. Biophys. Acta 776, 217–227), we describe the binding of a monoclonal anti-fluorescein antibody to a membrane bound fluorescein-lipid hapten. Those results suggest that some of the hapten fluorescein moiety is extended away from the membrane surface and is available for antibody binding, while some of the hapten is sequestered and not immediately available for antibody binding. In this paper, we carry out a spectroscopic study of the membrane-bound hapten and show that there is more than one physically distinct fluorophore environment, with the sequestered hapten associated with the phospholipid headgroup region. The amount of membrane-associated fluorophore depends upon the membrane lipid composition: most of the fluorophore is associated when the lipid is unsaturated or branched-chain phosphatidylcholines (PC), whereas the hapten is largely extended for PC/cholesterol mixtures. The effect of cholesterol on the availability of membrane-bound hapten to antibody binding is not unique to this system. The conversion between sequestered and extended hapten is slow (minutes).