Differential expression of ompA, ompB, pyk, nlpD and Cpn0585 genes between normal and interferon-γ treated cultures of Chlamydia pneumoniae
Differential expression of ompA, ompB, pyk, nlpD and Cpn0585 genes between normal and interferon-γ treated cultures of Chlamydia pneumoniae
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DOI：
10.1006/mpat.2000.0435
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发表时间：
2001-06-01
影响因子：
3.8
通讯作者：
Timms, P
中科院分区：
文献类型：
--
作者：
Mathews, S;George, C;Timms, P
许多衣原体感染的一个共同特点是，它们往往没有症状，如果不治疗，可能会持续很长一段时间。除了公认的衣原体发育周期的裂解阶段，现在出现的证据支持一个持续的阶段，在该周期中的网状体形态异常，可行的，但非传染性，大概也有改变基因表达模式。我们使用RT-PCR方法研究了14个基因（16 SrRNA、ompA、ompB、omcB、76 kDa、gseA、pmp 1、gltX、hsp 60、yaeT、pyk、nlpD、Cpn 0585、Cpn 1046）在正常和IFN-γ处理的肺炎衣原体细胞培养物之间的基因转录差异水平。即使在我们的IFN-γ处理的培养物中形态异常网状体的水平较低（约10%形态上可辨别，尽管推测更大的百分比处于持续状态但形态上尚未改变），我们鉴定了与正常培养物相比明显上调的五个基因（ompA、ompB、pyk、nlpD、Cpn 0585）。这种基因转录谱可能是衣原体一般应激状态的特征，在这种情况下由IFN-γ治疗诱导，但在其他体外和体内情况下可能更广泛地诱导。(C)北京：科学出版社.
A common feature of many chlamydial infections is that they are often asymptomatic and may persist for long periods of time if left untreated. In addition to the well recognized lytic stage of the chlamydial developmental cycle, evidence is now emerging to support a persistent phase in the cycle in which the reticulate bodies are morphologically abnormal, viable but non-infectious and presumably also have altered gene expression patterns. We used an RT-PCR approach to study the differential levels of gene transcription for 14 genes (16SrRNA, ompA, ompB, omcB, 76 kDa, gseA, pmp1, gltX, hsp60, yaeT, pyk, nlpD, Cpn0585, Cpn1046) between normal and IFN-gamma treated Chlamydia pneumoniae cell cultures. Even though the level of morphologically abnormal reticulate bodies in our IFN-gamma treated cultures was low (approximately 10% morphologically discernible, although presumably a larger percentage were in the persistent state but not yet morphologically altered) we identified five genes (ompA, ompB, pyk, nlpD, Cpn0585) that were clearly upregulated when compared to normal cultures. This gene transcript profile may be characteristic of a general stress state in Chlamydia, induced by IFN-gamma treatment in this case, but perhaps more widely induced in other in vitro and in vivo situations. (C) 2001 Academic Press.