Hepatocyte growth factor/scatter factor stimulates migration of muscle precursors in developing mouse tongue
Hepatocyte growth factor/scatter factor stimulates migration of muscle precursors in developing mouse tongue
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DOI：
10.1002/jcp.20056
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发表时间：
2004-11-01
影响因子：
5.6
通讯作者：
Daikuhara, Y
中科院分区：
文献类型：
--
作者：
Bandow, K;Ohnishi, T;Daikuhara, Y
肝细胞生长因子（HGF）在骨骼肌发育过程中刺激肌原细胞迁移。在小鼠中，HGF基因或其受体c-met的失活导致骨骼肌器官（如舌头）发育不全。碱性成纤维细胞生长因子（FGF-2）也诱导骨骼肌成肌细胞的迁移。通过比较HGF和FGF-2在骨骼肌发生中的作用，揭示了HGF在骨骼肌发育中的重要作用。与FGF-2不同，HGF诱导成肌细胞从发育中的小鼠舌迁移。通过比较HGF和FGF-2对胶原包被培养皿中培养的成肌细胞C2 C12中基质金属蛋白酶-9（MMP-9）表达的影响，确定HGF和FGF-2活性之间的差异。结果表明，HGF，而不是FGF-2，刺激MMP-9的表达，并通过激活磷脂酰肌醇3-激酶（PI 3 K），这是不相关的FGF-2信号转导介导的刺激。然而，这两种生长因子对C2 C12细胞中肌细胞生成素表达的减少和增殖产生几乎相同的作用，表明HGF而不是FGF-2在骨骼肌（包括舌头）的生成中起着至关重要的作用。此外，HGF通过PI 3 K信号通路的特定作用是诱导成肌细胞中MMP-9的表达和迁移。(C)2004 Wiley-Liss，Inc.
Hepatocyte growth factor (HGF) stimulates the migration of myogenic cells during the development of skeletal muscles. The inactivation of HGF genes or that of its receptor, c-met, in mice causes hypoplasia of skeletal muscle organs, such as the tongue. Basic fibroblast growth factor (FGF-2) also induces migration of skeletal myoblasts. A comparison of the functions of HGF and FGF-2 in myogenesis revealed the crucial effect of HGF in the development of skeletal muscles. Unlike FGF-2, HGF induced migration of myoblasts from the developing mouse tongue. The differences between the activities of HGF and FGF-2 were determined by comparing their effects on the expression of matrix metalloproteinase-9 (MMP-9) in myoblasts, C2C12 cells, cultured in collagen-coated dishes. The results showed that HGF, but not FGF-2, stimulated MMP-9 expression, and that the stimulation was mediated through the activation of phosphoinositide 3-kinase (PI3K) which was not associated with FGF-2 signal transduction. Nevertheless, both growth factors exerted almost the same effect on the reduction of myogenin expression in, and on the proliferation of, C2C12 cells, suggesting that HGF, rather than FGF-2, plays a crucial role in the generation of skeletal muscles, including the tongue. Moreover,the specific role of HGF through the PI3K signal pathway is the induction of MMP-9 expression in, and the migration of, myoblasts. (C) 2004 Wiley-Liss, Inc.