The prevalence of a microbiota in the digestive tract of Phlebotomus papatasi
The prevalence of a microbiota in the digestive tract of Phlebotomus papatasi
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DOI：
10.1080/00034983.1996.11813102
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发表时间：
1996-12-01
影响因子：
--
通讯作者：
Lane, RP
中科院分区：
文献类型：
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作者：
Dillon, RJ;ElKordy, E;Lane, RP
存在于许多昆虫物种的消化道中的细菌（Tanada和卡亚，1993）被认为在防止细菌和真菌昆虫病原体的定殖中是重要的（Dillon和Charnley，1991），并且可能干扰医学上重要的病原体的发展（Beier等人，1994; Pumpuni等人，1996年）。关于食血昆虫肠道微生物群流行的详细研究如此之少的一个原因是，人们假设血粉是“无菌的”，因此肠道微生物群不会发育。然而，血液并不是吸血昆虫的唯一食物。例如，白蛉，像其他一些吸血昆虫一样，经常服用来自许多来源的“糖粉”，包括叶子，水果和蚜虫蜜露，然后有足够的机会摄取污染微生物（Killick-Kendrick和Killick-Kendrick）。1987; Schlein和Yuval，1987;卡梅隆等人，1995年）。野生的成年帕帕塔白蛉也通常摄取植物组织的小块（Schlein和Jacobson，1994），其将包括通常存在于植物角质层上的一些微生物。Adler和Theodor（1929）是第一个提出其他微生物的存在可能会阻止利什曼原虫的发展的人。在白蛉的肠道里多年后，Schlein et al.（1985）记录了以色列帕帕塔斯利什曼原虫消化道中微生物污染的高发生率，并表明肠道污染可能干扰利什曼原虫传播。本研究的目的是调查的流行率的细菌感染的肠道野生捕捉Ph. papatasi和估计的人口规模的微生物存在。实验室饲养的白蛉也进行了检查，以确定细菌数量是否在实验室中发生变化。
The bacteria present in the digestive tracts of numerous insect species (Tanada and Kaya, 1993) are thought to be important in preventing colonization by bacterial and fungal entomopathogens (Dillon and Charnley, 1991) and may interfere with the development of medically important pathogens (Beier et al., 1994; Pumpuni et al., 1996). One reason why there have been so few detailed studies on the prevalence of gut microbiota in haematophagous insects is that it was assumed that bloodmeals are'sterile'and consequently a gut microbiota would not develop. However, blood is not the only meal taken by haematophagous insects. Phlebotomine sandflies, for example, like some other blood-sucking insects, often take a'sugarmeal'derived from a number of sources, including leaves, fruit and aphid honeydew, and then have ample opportunity to ingest contaminating microorganisms (Killick-Kendrick and Killick-Kendrick. 1987; Schlein and Yuval, 1987; Cameron et al., 1995). Wild, adult Phlebotomus papatasi also commonly ingest small pieces of plant tissue (Schlein and Jacobson, 1994) which will include some of the microorganisms normally present on plant cuticle. Adler and Theodor (1929) were the first to suggest that the presence of other microorganisms might prevent the development of Leishmania spp. in the sandfly gut. Many years later, Schlein et al.(1985) recorded a high incidence of microbial contamination in the digestive tract of Ph. papatasi from Israel and suggested that gut contamination might interfere with Leishmania transmission. The objective of the present study was to investigate the prevalence of bacterial infections in the guts of wild-caught Ph. papatasi and estimate the population size of the microorganisms present. Laboratory-reared sandflies were also examined to determine if the numbers of bacteria change in the laboratory.