Electroimmunodiffusion of 2 M, IgA and IgM in nanogram quantities with a hydroxyethylcellulose-agarose gel: application to unconcentrated CSF.
Electroimmunodiffusion of 2 M, IgA and IgM in nanogram quantities with a hydroxyethylcellulose-agarose gel: application to unconcentrated CSF.
复制标题
使用羟乙基纤维素-琼脂糖凝胶对纳克量的 2 M、IgA 和 IgM 进行电免疫扩散：应用于未浓缩的 CSF。
DOI：
10.1016/0009-8981(72)90368-3
复制
发表时间：
1972
期刊：
影响因子：
--
通讯作者：
L. Tömpe
中科院分区：
文献类型：
--
作者：
E. Schuller;M. Lefèvre;L. Tömpe
一种新的凝胶（羟乙基纤维素和琼脂糖的混合物）可以通过电免疫扩散来测定高分子量蛋白质（如α2-巨球蛋白），弱电泳迁移率蛋白质（如IgA）或呈现这两种不便的蛋白质（如IgM）。所得灵敏度达到0.10 ~ 0.15 mg/100 ml （5 ~ 7.5 ng）。讨论了未浓缩脑脊液的初步结果，特别是在脱髓鞘疾病中。该方法易于应用于血清（例如：α2M），方便稀释后：它允许关联，同时，血清和脑脊液中的蛋白质水平。
A new gel (mixture of hydroxyethylcellulose and agarose) allows, by electroimmunodiffusion, the determination of high molecular weight proteins (asα2-macroglobulin), weak electrophoretic mobility proteins (like IgA) or proteins presenting these two inconvenients (IgM, for example). The sensitivity obtained reaches 0.10 to 0.15 mg/100 ml (5 to 7.5 ng). The preliminary results, on unconcentrated CSF, are discussed, especially in demyelinating diseases. The method is easily applied to serum (example:α2M) after convenient dilution: it allows to correlate, at the same time, the level of a protein in serum and CSF.