Quantitative studies on the iodinated components secreted by the rat thyroid gland as determined by in situ perfusion.
Quantitative studies on the iodinated components secreted by the rat thyroid gland as determined by in situ perfusion.
复制标题
原位灌注测定大鼠甲状腺分泌的碘化成分的定量研究。
DOI：
10.1210/endo-81-5-946
复制
发表时间：
1967
期刊：
影响因子：
4.8
通讯作者：
Monte A. Greer
中科院分区：
文献类型：
--
作者：
Kenjiro Inoue;Yvonne Grimm;Monte A. Greer
描述了一种原位灌注大鼠甲状腺并分析甲状腺静脉流出物的技术。该程序可以通过避免再循环放射性物质的污染来测定预先标记的甲状腺分泌的放射性成分。在将血液用于灌注之前用涤纶或玻璃棉过滤血液大大减少了滤泡内的出血以及随后在甲状腺流出物中出现的甲状腺球蛋白。灌注前 24 小时给予 125I 和灌注前 10 分钟给予 131I 进行双重标记，可以对同一动物中在不同时间间隔进入甲状腺的碘标记材料进行分析。以 12.5 mU/ml 的浓度添加到灌注血液中的 TSH 显着增加了 24 小时前用 125I 标记的大鼠中碘甲腺原氨酸和碘化物的分泌以及 T3 + T4/I− 比率。 TSH 还诱导 131I 急性标记大鼠碘甲状腺原氨酸分泌增加。 125I 和... 的 T3/T4 比
A technique is described for perfusing the rat thyroid gland in situ and analyzing the effluent from the thyroid vein. This procedure allows determination of radioactive components secreted from prelabeled thyroid glands by avoiding contamination from recirculating radioactive materials. Filtration of blood with Dacron or glass wool before itsi use in perfusion greatly reduced hemorrhage into the follicles and the consequent appearance of thyroglobulin in the thyroid effluent. Doublelabeling with 125I given 24 hr before and 131I given 10 min before perfusion permitted analysis of materials labeled with iodine entering the thyroid gland at these different time intervals in the same animal. TSH added to the perfusing blood at a concentration of 12.5 mU/ml markedly increased the secretion of iodothyronines and of iodide and the T3 + T4/I− ratio in rats labeled 24 hr previously with 125I. TSH also induced an increase in iodothyronine secretion in rats acutely labeled with 131I. The T3/T4 ratio of both 125I an...