Platelet-derived growth factor-BB accelerates wound closure in a new mesentery culture model without macrophages.
Platelet-derived growth factor-BB accelerates wound closure in a new mesentery culture model without macrophages.
复制标题
血小板衍生生长因子-BB 在无巨噬细胞的新型肠系膜培养模型中加速伤口闭合。
DOI：
10.1097/00000637-199408000-00006
复制
发表时间：
1994
影响因子：
1.5
通讯作者：
Mustoe,TA
中科院分区：
文献类型：
--
作者：
Wu,L;Kumar,SB;Porras-Reyes,BH;Gramates,PH;Mustoe,TA
血小板衍生生长因子-BB在体外具有多种作用，并已在几种动物模型中证明可加速伤口愈合，但其体内确切的作用机制尚未得到充分解释。建立大鼠肠系膜培养模型，并将血小板衍生生长因子-BB（100 ng/ml）加入到含有2%胎牛血清的培养基中以检查其封闭效果。对肠系膜标本进行常规苏木素和伊红染色以及巨噬细胞免疫组化染色。与对照组相比，加入血小板衍生生长因子-BB后，细胞的闭合率和完全闭合率显著提高（p< 0.05）。体外样本中的巨噬细胞明显少于体内样本。总之，血小板源性生长因子-BB可以有助于伤口闭合肠系膜缺损，即使没有巨噬细胞，这表明第二种机制，它可以直接作用于间充质细胞在伤口愈合。这种组织培养模型对于进一步了解生长因子相互作用和伤口愈合是有用的。
Platelet-derived growth factor-BB has multiple effects in vitro and has been demonstrated to accelerate wound healing in several animal models, but its precise mechanisms of action in vivo have not been fully explained. A rat mesentery culture model was developed, and platelet-derived growth factor-BB (100 ng/ml) was added to the medium containing 2% fetal calf serum for examining its closure effects. The mesentery specimens were stained by routine hematoxylin and eosin and immunohistochemical staining for macrophages. A significant improvement in closure rate and complete closure rate was seen when plateletderived growth factor-BB was added to the medium compared with the controls (p< 0.05). There are significantly fewer macrophages in the in vitro specimen than in the in vivo specimen. In conclusion, platelet-derived growth factor-BB can contribute to wound closure of a mesenteric defect even without macrophages, suggesting a second mechanism in which it can act directly on mesenchymal cells during wound healing. This tissue-culture model is useful for gaining further insights into growth factor interactions and wound healing.