Analysis of the genome sequence of an alpaca coronavirus
Analysis of the genome sequence of an alpaca coronavirus
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DOI：
10.1016/j.virol.2007.03.035
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发表时间：
2007-08-15
期刊：
影响因子：
3.7
通讯作者：
Rohrmann, G. F.
中科院分区：
文献类型：
--
作者：
Jin, L.;Cebra, C. K.;Rohrmann, G. F.
新大陆骆驼冠状病毒感染于1998年在患有严重腹泻的骆驼和羊驼中首次发现。为了了解这种感染，对其中一株冠状病毒分离株进行了测序和分析。它的基因组长31,076个核苷酸，包括3‘端的聚A尾。该病毒命名为ACoV-00-1381(ACoV)，编码2组牛冠状病毒(BCOV)特有的全部10个开放阅读框(ORF)。系统进化分析表明，ACoV基因组与两个BCOV株ENT和LN紧密聚在一起(>；99.5%)，并与其他BCOV株(Mebus，魁北克，DB2.)，人冠状病毒(株043)(>；96%)和猪疱疹凝集性脑脊髓炎病毒(>；93%)有密切的亲缘关系。共有145个点突变和1个核苷酸缺失与BCOV ENT相关。大多数开放阅读框架高度保守，但所预测的Spike蛋白(S)与牛冠状病毒LN和ENT分别有9个和12个氨基酸的差异，并且显示出比其他蛋白更多的相对数量的变化。系统发育分析表明，ACoV与BCOV ENT和LN具有相同的祖先。(C)2007 Elsevier Inc.保留所有权利。
Coronaviral infection of New World camelids was first identified in 1998 in llamas and alpacas with severe diarrhea. In order to understand this infection, one of the coronavirus isolates was sequenced and analyzed. It has a genome of 31,076 nt including the poly A tail at the 3' end. This virus designated as ACoV-00-1381 (ACoV) encodes all 10 open reading frames (ORFs) characteristic of Group 2 bovine coronavirus (BCoV). Phylogenetic analysis showed that the ACoV genome is clustered closely (> 99.5% identity) with two BCoV strains, ENT and LUN, and was also closely related to other BCoV strains (Mebus, Quebec, DB2), a human corona virus (strain 043) (>96%), and porcine hernagglutinating encephalomyelitis virus (> 93% identity). A total of 145 point mutations and one nucleotide deletion were found relative to the BCoV ENT. Most of the ORFs were highly conserved; however, the predicted spike protein (S) has 9 and 12 amino acid differences from BCoV LUN and ENT, respectively, and shows a higher relative number of changes than the other proteins. Phylogenetic analysis suggests that ACoV shares the same ancestor as BCoV ENT and LUN. (c) 2007 Elsevier Inc. All rights reserved.