Correlation between chloroplast ultrastructure and chlorophyll fluorescence characteristics in the leaves of rice (Oryza sativa L.) grown under salinity
Correlation between chloroplast ultrastructure and chlorophyll fluorescence characteristics in the leaves of rice (Oryza sativa L.) grown under salinity
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DOI：
10.1626/pps.11.139
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发表时间：
2008-01-01
影响因子：
2.5
通讯作者：
Miyake, Hiroshi
中科院分区：
文献类型：
--
作者：
Yamane, Koji;Kawasaki, Michio;Miyake, Hiroshi
水稻幼苗。CV.用75、100、150和200 mM NaCl分别处理水培3周的日本晴14、14、6和3天，并检查记录叶绿素荧光的区域的叶绿体超微结构。NaCl处理降低了叶绿素荧光产率的比值（F（v）/F（m）），导致类囊体肿胀。通过肿胀的类囊体长度占类囊体总长度的百分比来量化类囊体的肿胀。该值随着NaCl浓度的增加而增加。虽然F（v）/F（m）在所有浓度的NaCl下都降低，但用75或100 mM NaCl处理时，最小荧光产率F没有增加。在75和100 mM NaCl下，溶胀长度的百分比较低。另一方面，F（0）增加，类囊体的肿胀是显着的150和200 mM NaCl处理。这些结果表明，盐胁迫下F（0）的增加导致F（v）/F（m）的降低，这与盐胁迫引起的超微结构损伤有关。由于F（0）的增加而导致的F（v）/F（m）的降低预计可用作评估盐胁迫下叶绿体（尤其是类囊体膜）损伤的指标。
The seedlings of Oryza saliva L. cv. Nipponbare grown by hydroponic culture for 3 wks were treated with 75, 100, 150 and 200 mM NaCl for 14, 14, 6 and 3 days, respectively, and examined for chloroplast ultrastructure in the region where chlorophyll fluorescence had been recorded. NaCl treatment decreased the ratio of variable to maximum chlorophyll fluorescence yield (F(v)/F(m)) and caused swelling of thylakoids. The swelling of thylakoids was quantified by the percentage of the length of swollen thylakoids to the total length of thylakoids. This value was increased with increasing NaCl concentration. Although F(v)/F(m) decreased at all concentrations of NaCl, the minimal fluorescence yield F, was not increased by the treatment with 75 or 100 mM NaCl. The percentage of the length of swelling was low at 75 and 100 mM NaCl. On the other hand, F(0) increased and the swelling of thylakoids was prominent with 150 and 200 mM NaCl treatment. These results suggest that the decrease in F(v)/F(m) due to the increase in F(0) under salt stress correlates with the ultrastructural damage. The decrease in F(v)/F(m) due to the increase in F(0) is expected to be useful as an indicator to evaluate the damage in chloroplasts, especially in thylakoid membranes, under salinity.